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- Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis
- Min-hee Hong; Jungoh Ahn; S Yoo; J Hong; Eun Gyo Lee; I Yoon; Joon Ki Jung; Hong-Weon Lee
- Bibliographic Citation
- Veterinary Microbiology, vol. 148, no. 1, pp. 89-92
- Publication Year
- Haemophilus parasuis causes contagious porcine Glasser's disease, which is occurring worldwide and leads to severe losses in the pig industry. To identify novel antigen candidates against this disease, 22 surface-exposed or secreted proteins were selected from the annotated H. parasuis genome by reverse vaccinology strategy. Expression of these proteins in Escherichia coli was attempted. Immunogenicity of the expressed candidates was assessed using Western blot analysis with mouse-derived antiserum prepared with whole bacteria of H. parasuis serovar 4 or 5. Three ABC-type transporters (OppA, YfeA and PlpA) and 1 curli protein assembly (CsgG) were identified as potent immunogenic proteins. The proteins show cross-reactions when tested with sera raised against serovars 4 and 5 of H parasuis.
- Antigenic protein; Haemophilus parasuis; Reverse vaccinology
- Appears in Collections:
- Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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