Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis

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Title
Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis
Author(s)
Min-hee Hong; Jungoh Ahn; S Yoo; J Hong; Eun Gyo Lee; I Yoon; Joon Ki Jung; Hong-Weon Lee
Bibliographic Citation
Veterinary Microbiology, vol. 148, no. 1, pp. 89-92
Publication Year
2011
Abstract
Haemophilus parasuis causes contagious porcine Glasser's disease, which is occurring worldwide and leads to severe losses in the pig industry. To identify novel antigen candidates against this disease, 22 surface-exposed or secreted proteins were selected from the annotated H. parasuis genome by reverse vaccinology strategy. Expression of these proteins in Escherichia coli was attempted. Immunogenicity of the expressed candidates was assessed using Western blot analysis with mouse-derived antiserum prepared with whole bacteria of H. parasuis serovar 4 or 5. Three ABC-type transporters (OppA, YfeA and PlpA) and 1 curli protein assembly (CsgG) were identified as potent immunogenic proteins. The proteins show cross-reactions when tested with sera raised against serovars 4 and 5 of H parasuis.
Keyword
Antigenic proteinHaemophilus parasuisReverse vaccinology
ISSN
0378-1135
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.vetmic.2010.07.023
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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