Isolation of hyperthermal stress responsive genes in soft coral (Scleronephthya gracillimum)

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Title
Isolation of hyperthermal stress responsive genes in soft coral (Scleronephthya gracillimum)
Author(s)
S Woo; H Y Jeon; J Lee; J I Song; Hong-Seog Park; S Yum
Bibliographic Citation
Molecular & Cellular Toxicology, vol. 6, no. 4, pp. 387-393
Publication Year
2010
Abstract
The extensive isolation of genes responsive to hyperthermal stress conditions in soft coral (Scleronephthya gracillimum) is described. Soft coral colonies were exposed to high seawater temperature conditions. Gene candidates whose transcript levels changed in response to hyperthermal conditions were identified by differential display polymerase chain reaction (DD-PCR). Twenty-four types of candidate genes were identified, 18 of which were upregulated in expression and 6 of which were downregulated. The genes were found to function in post-translational modification, protein turnover and chaperones (O); translation, ribosomal structure and biogenesis (J); signal transduction mechanisms (T); defense mechanisms (V); inorganic ion transport and metabolism (P); energy production and conversion (C); cytoskeleton (Z); cell cycle control, cell division and chromosome partitioning (D); lipid transport and metabolism (I); chromatin structure and dynamics (B); transcription (K); replication, recombination and repair (L); secondary metabolites biosynthesis, transport and catabolism (Q); extracellular structures (W); general function prediction (R); and finally, unknown function (S) based on KOG classification. Among these candidates, their expressional changes were confirmed by real-time quantitative PCR (qRT-PCR). These 24 isolated gene candidates were differentially expressed and therefore have great potential as molecular biomarkers for the identification of environmental stressors.
Keyword
Differentially expressed genesHyperthermal stressReal-time quantitative PCRScleronephthya gracillimumSoft coral
ISSN
I000-0184
Publisher
South Korea
DOI
http://dx.doi.org/10.1007/s13273-010-0051-0
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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