Detection of low-abundant novel transcripts in mouse hematopoietic stem cells

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dc.contributor.authorH S Kim-
dc.contributor.authorJ Hwang-
dc.contributor.authorY H Kim-
dc.contributor.authorS Kim-
dc.contributor.authorJ W Lee-
dc.contributor.authorH S Kang-
dc.contributor.authorK S Kim-
dc.contributor.authorJ H Ha-
dc.contributor.authorJ W Chung-
dc.contributor.authorKyu Tae Chang-
dc.contributor.authorZ Y Ryoo-
dc.contributor.authorS Lee-
dc.date.accessioned2017-04-19T09:22:05Z-
dc.date.available2017-04-19T09:22:05Z-
dc.date.issued2009-
dc.identifier.issn0026-8925-
dc.identifier.uri10.1007/s00438-009-0469-zko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10021-
dc.description.abstractGene expression profiles of hematopoietic stem cells (HSCs) provide clues for understanding molecular mechanisms of HSC behavior, including self-renewal and differentiation. We took advantage of serial analysis of gene expression (SAGE) to identify medium- and low-abundant transcripts expressed in HSCs/hematopoietic progenitor cells (HPCs). Among a total of 31,380 unique transcripts, 17,326 (55%) correspond to known genes and, 14,054 (45%) are low-copy transcripts that have no matches to currently known genes. Among the former class, 3,899 (23%) were alternatively spliced transcripts and 3,754 (22%) represent anti-sense transcripts from known genes. Mapping of the SAGE tags to the mouse genome showed that differences in gene expression exist among chromosomes. In addition, comparison of the HSCs/HPCs SAGE data to that of myeloid progenitor cells revealed that massive genetic reprogramming occurs in hematopoietic cell differentiation. Our results demonstrate a previously unrecognized complexity of gene expression in HSCs/HPCs, and indicate the need for further efforts to fully identify and characterize the transcripts expressed in this cell type.-
dc.publisherSpringer-
dc.titleDetection of low-abundant novel transcripts in mouse hematopoietic stem cells-
dc.title.alternativeDetection of low-abundant novel transcripts in mouse hematopoietic stem cells-
dc.typeArticle-
dc.citation.titleMolecular Genetics and Genomics-
dc.citation.number4-
dc.citation.endPage370-
dc.citation.startPage363-
dc.citation.volume282-
dc.contributor.affiliatedAuthorKyu Tae Chang-
dc.contributor.alternativeName김형수-
dc.contributor.alternativeName황준모-
dc.contributor.alternativeName김영훈-
dc.contributor.alternativeName김성건-
dc.contributor.alternativeName이재웅-
dc.contributor.alternativeName강형식-
dc.contributor.alternativeName김길수-
dc.contributor.alternativeName하지홍-
dc.contributor.alternativeName정진웅-
dc.contributor.alternativeName장규태-
dc.contributor.alternativeName류재영-
dc.contributor.alternativeName이상규-
dc.identifier.bibliographicCitationMolecular Genetics and Genomics, vol. 282, no. 4, pp. 363-370-
dc.identifier.doi10.1007/s00438-009-0469-z-
dc.subject.keywordGene expression-
dc.subject.keywordGr-1 myeloid progenitor cell-
dc.subject.keywordHematopoietic stem cell-
dc.subject.keywordSAGE-
dc.subject.keywordTranscriptome-
dc.subject.localGene Expression-
dc.subject.localGene expression-
dc.subject.localgene expression-
dc.subject.localGr-1 myeloid progenitor cell-
dc.subject.localHematopoietic stem cell-
dc.subject.localhematopoietic stem cell-
dc.subject.localHematopoietic stem cells-
dc.subject.localSAGE-
dc.subject.localTranscriptomes-
dc.subject.localtranscriptome-
dc.subject.localTranscriptome-
dc.description.journalClassY-
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