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- Use of terminal restriction length polymorphism (T-RFLP) analysis to evaluate uncultivable microbial community structure of soil = T-RFLP 분석을 이용한 토양미생물군집구조 평가
- P S Chauhan; C C Shagol; W Yim; S C Tipayno; Chang-Gi Kim; T Sa
- Bibliographic Citation
- Korean Journal of Soil Science & Fertilizer, vol. 44, no. 1, pp. 127-145
- Publication Year
- Various environmental ecosystems are valuable sources for microbial ecology studies, and their analyses
using recently developed molecular ecological approaches have drawn significant attention within the
scientific community. Changes in the microbial community structures due to various anthropogenic activities
can be evaluated by various culture-independent methods e.g. ARISA, DGGE, SSCP, T-RFLP, clone library,
pyrosequencing, etc. Direct amplification of total community DNA and amplification of most conserved
region (16S rRNA) are common initial steps, followed by either fingerprinting or sequencing analysis.
Fingerprinting methods are relatively quicker than sequencing analysis in evaluating the changes in the
microbial community. Being an efficient, sensitive and time- and cost effective method, T-RFLP is regularly
used by many researchers to access the microbial diversity. Among various fingerprinting methods T-RFLP
became an important tool in studying the microbial community structure because of its sensitivity and
reproducibility. In this present review, we will discuss the important developments in T-RFLP methodology to
distinguish the total microbial diversity and community composition in the various ecosystems.
- Microbial diversityCommunity structureT-RFLP16S rRNA
- Korea Soc-Assoc-Inst
- Appears in Collections:
- Division of Bio Technology Innovation > Bio-Evaluation Center > 1. Journal Articles
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