Use of terminal restriction length polymorphism (T-RFLP) analysis to evaluate uncultivable microbial community structure of soil = T-RFLP 분석을 이용한 토양미생물군집구조 평가

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Title
Use of terminal restriction length polymorphism (T-RFLP) analysis to evaluate uncultivable microbial community structure of soil = T-RFLP 분석을 이용한 토양미생물군집구조 평가
Author(s)
P S Chauhan; C C Shagol; W Yim; S C Tipayno; Chang-Gi Kim; T Sa
Bibliographic Citation
Korean Journal of Soil Science & Fertilizer, vol. 44, no. 1, pp. 127-145
Publication Year
2011
Abstract
Various environmental ecosystems are valuable sources for microbial ecology studies, and their analyses using recently developed molecular ecological approaches have drawn significant attention within the scientific community. Changes in the microbial community structures due to various anthropogenic activities can be evaluated by various culture-independent methods e.g. ARISA, DGGE, SSCP, T-RFLP, clone library, pyrosequencing, etc. Direct amplification of total community DNA and amplification of most conserved region (16S rRNA) are common initial steps, followed by either fingerprinting or sequencing analysis. Fingerprinting methods are relatively quicker than sequencing analysis in evaluating the changes in the microbial community. Being an efficient, sensitive and time- and cost effective method, T-RFLP is regularly used by many researchers to access the microbial diversity. Among various fingerprinting methods T-RFLP became an important tool in studying the microbial community structure because of its sensitivity and reproducibility. In this present review, we will discuss the important developments in T-RFLP methodology to distinguish the total microbial diversity and community composition in the various ecosystems.
Keyword
Microbial diversityCommunity structureT-RFLP16S rRNA
ISSN
0367-6315
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.7745/KJSSF.2011.44.1.127
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Bio-Evaluation Center > 1. Journal Articles
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