Sequential conjugation of 6-aminohexanoic acids and L-arginines to poly(amidoamine) dendrimer to modify hydrophobicity and flexibility of the polymeric gene carrier

Cited 6 time in scopus
Metadata Downloads
Title
Sequential conjugation of 6-aminohexanoic acids and L-arginines to poly(amidoamine) dendrimer to modify hydrophobicity and flexibility of the polymeric gene carrier
Author(s)
G S Yu; H N Yu; Y H Choe; S J Son; Tai-Hwan Ha; J S Choi
Bibliographic Citation
Bulletin of Korean Chemical Society, vol. 32, no. 2, pp. 651-655
Publication Year
2011
Abstract
We synthesized a novel cationic dendrimer consisting of a poly(amidoamine) dendrimer (PAMAM, generation 4) backbone with both L-arginine (Arg) at the termini and 6-aminohexanoic acid (Ahx) between the original core polymer and the peripheral Arg units. The sequential chemical modification of PAMAM G4 with Ahx and Arg resulted in higher transfection efficiency with much less cytotoxicity. PAMAM G4-Ahx-Arg formed stable polyplexes at weight ratios of 8:1 or higher (polymer: plasmid DNA), and the mean polyplex diameter was 180 ± 20 nm. PAMAM G4-Ahx-Arg showed much higher transfection ability than PAMAM G4 or PAMAM G4-Ahx. Furthermore, PAMAM G4-Ahx-Arg was much less cytotoxic than PEI25KD and PAMAM G4-Arg. In addition to Arg grafting of the PAMAM dendrimer, which endows a higher transfection capability, the addition of Ahx spacer increased dendrimer hydrophobicity, introduced flexibility into the conjugated amino acids, and reduced cytotoxicity. Overall, it appears that the concomitant modification of PAMAM with Ahx and Arg could lead to new PAMAM conjugates with better performances.
Keyword
DendrimerGene deliveryPolyplexTransfection
ISSN
0253-2964
Publisher
Wiley
DOI
http://dx.doi.org/10.5012/bkcs.2011.32.2.651
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.