Transgenic poplar expressing Arabidopsis NDPK2 enhances growth as well as oxidative stress tolerance

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Title
Transgenic poplar expressing Arabidopsis NDPK2 enhances growth as well as oxidative stress tolerance
Author(s)
Yun Hee Kim; Myoung Duck Kim; Y I Choi; Sung Chul Park; D J Yun; E W Noh; Haeng Soon Lee; Sang Soo Kwak
Bibliographic Citation
Plant Biotechnology Journal, vol. 9, no. 3, pp. 334-347
Publication Year
2011
Abstract
Nucleoside diphosphate kinase 2 (NDPK2) is known to regulate the expression of antioxidant genes in plants. Previously, we reported that overexpression of Arabidopsis NDPK2 (AtNDPK2) under the control of an oxidative stress-inducible SWPA2 promoter in transgenic potato and sweetpotato plants enhanced tolerance to various abiotic stresses. In this study, transgenic poplar (Populus alba×Poplus glandulosa) expressing the AtNDPK2 gene under the control of a SWPA2 promoter (referred to as SN) was generated to develop plants with enhanced tolerance to oxidative stress. The level of AtNDPK2 expression and NDPK activity in SN plants following methyl viologen (MV) treatment was positively correlated with the plant's tolerance to MV-mediated oxidative stress. We also observed that antioxidant enzyme activities such as ascorbate peroxidase, catalase and peroxidase were increased in MV-treated leaf discs of SN plants. The growth of SN plants was substantially increased under field conditions including increased branch number and stem diameter. SN plants exhibited higher transcript levels of the auxin-response genes IAA2 and IAA5. These results suggest that enhanced AtNDPK2 expression affects oxidative stress tolerance leading to improved plant growth in transgenic poplar.
Keyword
Antioxidant enzymeAuxinNucleoside diphosphate kinase 2Stress-inducible promoterTransgenic poplar
ISSN
1467-7644
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1467-7652.2010.00551.x
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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