A highly active endo-beta-1,4-mannanase produced by Cellulosimicrobium sp. strain HY-13, a hemicellulolytic bacterium in the gut of Eisenia fetida

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dc.contributor.authorDo Young Kim-
dc.contributor.authorS J Ham-
dc.contributor.authorHyun Ju Lee-
dc.contributor.authorY J Kim-
dc.contributor.authorD H Shin-
dc.contributor.authorY H Rhee-
dc.contributor.authorKwang Hee Son-
dc.contributor.authorHo Yong Park-
dc.date.accessioned2017-04-19T09:22:10Z-
dc.date.available2017-04-19T09:22:10Z-
dc.date.issued2011-
dc.identifier.issn0141-0229-
dc.identifier.uri10.1016/j.enzmictec.2010.12.013ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10046-
dc.description.abstractA xylanolytic gut bacterium isolated from Eisenia fetida, Cellulosimicrobium sp. strain HY-13, produced an extracellular glycoside hydrolase capable of efficiently degrading mannose-based substrates such as locust bean gum, guar gum, mannotetraose, and mannopentaose. The purified mannan-degrading enzyme (ManK, 34,926 Da) from strain HY-13 was found to have an N-terminal amino acid sequence of DEATTDGLHVVDD, which has not yet been identified. Under the optimized reaction conditions of 50 °C and pH 7.0, ManK exhibited extraordinary high specific activities of 7109 IU/mg and 5158 IU/mg toward locust bean gum and guar gum, respectively, while the enzyme showed no effect on sugars substituted with p-nitrophenol and various non-mannose carbohydrates. Thin layer chromatography revealed that the enzyme degraded locust bean gum to mannobiose and mannotetraose. No detectable amount of mannose was produced from hydrolytic reactions with the substrates. ManK strongly attached to Avicel, β-cyclodextrin, lignin, and poly(3-hydroxybutyrate) granules, but not bound to chitin, chitosan, curdlan, or insoluble oat spelt xylan. The aforementioned characteristics of ManK suggest that it is a unique endo-β-1,4-mannanase without additional carbohydrolase activities, which differentiates it from other well-known carbohydrolases.-
dc.publisherElsevier-
dc.titleA highly active endo-beta-1,4-mannanase produced by Cellulosimicrobium sp. strain HY-13, a hemicellulolytic bacterium in the gut of Eisenia fetida-
dc.title.alternativeA highly active endo-beta-1,4-mannanase produced by Cellulosimicrobium sp. strain HY-13, a hemicellulolytic bacterium in the gut of Eisenia fetida-
dc.typeArticle-
dc.citation.titleEnzyme and Microbial Technology-
dc.citation.number4-
dc.citation.endPage370-
dc.citation.startPage365-
dc.citation.volume48-
dc.contributor.affiliatedAuthorDo Young Kim-
dc.contributor.affiliatedAuthorHyun Ju Lee-
dc.contributor.affiliatedAuthorKwang Hee Son-
dc.contributor.affiliatedAuthorHo Yong Park-
dc.contributor.alternativeName김도영-
dc.contributor.alternativeName함수진-
dc.contributor.alternativeName이현주-
dc.contributor.alternativeName김이준-
dc.contributor.alternativeName신동하-
dc.contributor.alternativeName이영하-
dc.contributor.alternativeName손광희-
dc.contributor.alternativeName박호용-
dc.identifier.bibliographicCitationEnzyme and Microbial Technology, vol. 48, no. 4, pp. 365-370-
dc.identifier.doi10.1016/j.enzmictec.2010.12.013-
dc.subject.keywordCellulosimicrobium sp. strain HY-13-
dc.subject.keywordEisenia fetida-
dc.subject.keywordGut bacterium-
dc.subject.keywordHighly active endo-β-1,4-mannanase-
dc.subject.keywordMannan-degrading enzyme-
dc.subject.localcellulosimicrobium sp. HY-13-
dc.subject.localCellulosimicrobium sp. strain HY-13-
dc.subject.localeisenia fetida-
dc.subject.localEisenia fetida-
dc.subject.localGut bacterium-
dc.subject.localHighly active endo-β-1,4-mannanase-
dc.subject.localMannan-degrading enzyme-
dc.description.journalClassY-
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Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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