Upregulation of RhoB via c-Jun N-terminal kinase signaling induces apoptosis of the human gastric carcinoma NUGC-3 cells treated with NSC12618

Cited 20 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorBo Kyung Kim-
dc.contributor.authorHwan Mook Kim-
dc.contributor.authorKyung Sook Chung-
dc.contributor.authorD M Kim-
dc.contributor.authorSong Kyu Park-
dc.contributor.authorA Song-
dc.contributor.authorKyoung Jae Won-
dc.contributor.authorKiho Lee-
dc.contributor.authorY K Oh-
dc.contributor.authorK Lee-
dc.contributor.authorK B Song-
dc.contributor.authorJ A Simon-
dc.contributor.authorG Han-
dc.contributor.authorMi Sun Won-
dc.date.accessioned2017-04-19T09:22:10Z-
dc.date.available2017-04-19T09:22:10Z-
dc.date.issued2011-
dc.identifier.issn01433334-
dc.identifier.uri10.1093/carcin/bgq244ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10047-
dc.description.abstractRhoB expression is reduced in most invasive tumors, with loss of RhoB expression correlating significantly with tumor stage. Here, we demonstrate that upregulation of RhoB by the potent anticancer agent NSC126188 induces apoptosis of NUGC-3 human gastric carcinoma cells. The crucial role of RhoB in NSC126188-induced apoptosis is indicated by the rescue of NUGC-3 cells from apoptosis by knockdown of RhoB. In the presence of NSC126188, c-Jun N-terminal kinase (JNK) signaling was activated, and the JNK inhibitor SP600125 reduced RhoB expression and suppressed the apoptosis of NUGC-3 cells. Knockdowns of mitogen-activated protein kinase kinase (MKK) 4/7, JNK1/2 and c-Jun downregulated RhoB expression and rescued cells from apoptotic death in the presence of NSC126188. The JNK inhibitor SP600125 suppressed transcriptional activation of RhoB in the presence of NSC126188, as indicated by a reporter assay that used luciferase under the RhoB promoter. The ability of NSC126188 to increase luciferase activity through both the p300-binding site and the inverted CCAAT sequence (iCCAAT box) suggests that JNK signaling to upregulate RhoB expression is mediated through both the p300-binding site and the iCCAAT box. However, the JNK inhibitor SP600125 did not inhibit the upregulation of RhoB by farnesyltransferase inhibitor (FTI)-277. The p300-binding site did not affect activation of the RhoB promoter by FTI-277 in NUGC-3 cells, suggesting that the transcriptional activation of RhoB by NSC126188 occurs by a different mechanism than that reported for FTIs. Our data indicate that NSC126188 increases RhoB expression via JNK-mediated signaling through a p300-binding site and iCCAAT box resulting in apoptosis of NUGC-3 cells.-
dc.publisherOxford Univ Press-
dc.titleUpregulation of RhoB via c-Jun N-terminal kinase signaling induces apoptosis of the human gastric carcinoma NUGC-3 cells treated with NSC12618-
dc.title.alternativeUpregulation of RhoB via c-Jun N-terminal kinase signaling induces apoptosis of the human gastric carcinoma NUGC-3 cells treated with NSC12618-
dc.typeArticle-
dc.citation.titleCarcinogenesis-
dc.citation.number3-
dc.citation.endPage261-
dc.citation.startPage254-
dc.citation.volume32-
dc.contributor.affiliatedAuthorBo Kyung Kim-
dc.contributor.affiliatedAuthorKyung Sook Chung-
dc.contributor.affiliatedAuthorMi Sun Won-
dc.contributor.alternativeName김보경-
dc.contributor.alternativeName김환묵-
dc.contributor.alternativeName정경숙-
dc.contributor.alternativeName김동명-
dc.contributor.alternativeName박성규-
dc.contributor.alternativeName-
dc.contributor.alternativeName원경재-
dc.contributor.alternativeName이기호-
dc.contributor.alternativeName오유경-
dc.contributor.alternativeName이경-
dc.contributor.alternativeName송경빈-
dc.contributor.alternativeNameSimon-
dc.contributor.alternativeName한균희-
dc.contributor.alternativeName원미선-
dc.identifier.bibliographicCitationCarcinogenesis, vol. 32, no. 3, pp. 254-261-
dc.identifier.doi10.1093/carcin/bgq244-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Division of Research on National Challenges > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.