Essential role of YlMPO1, a novel Yarrowia lipolytica homologue of Saccharomyces cerevisiae MNN4, in mannosylphosphorylation of N- and O-linked glycans

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dc.contributor.authorJ N Park-
dc.contributor.authorY Song-
dc.contributor.authorS A Cheon-
dc.contributor.authorOh Suk Kwon-
dc.contributor.authorDoo-Byoung Oh-
dc.contributor.authorY Jigami-
dc.contributor.authorJ Y Kim-
dc.contributor.authorH A Kang-
dc.date.accessioned2017-04-19T09:22:10Z-
dc.date.available2017-04-19T09:22:10Z-
dc.date.issued2011-
dc.identifier.issn0099-2240-
dc.identifier.uri10.1128/AEM.02323-10ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10048-
dc.description.abstractMannosylphosphorylation of N- and O-glycans, which confers negative charges on the surfaces of cells, requires the functions of both MNN4 and MNN6 in Saccharomyces cerevisiae. To identify genes relevant to mannosylphosphorylation in the dimorphic yeast Yarrowia lipolytica, the molecular functions of five Y. lipolytica genes showing significant sequence homology with S. cerevisiae MNN4 and MNN6 were investigated. A set of mutant strains in which Y. lipolytica MNN4 and MNN6 homologues were deleted underwent glycan structure analysis. In contrast to S. cerevisiae MNN4 (ScMNN4), the Y. lipolytica MNN4 homologue, MPO1 (YlMPO1), encodes a protein that lacks the long KKKKEEEE repeat domain at its C terminus. Moreover, just a single disruption of YlMPO1 resulted in complete disappearance of the acidic sugar moiety in both the N- and O-linked glycan profiles. In contrast, even quadruple disruption of all ScMNN6 homologues, designated YlKTR1, YlKTR2, YlKTR3, and YlKTR4, resulted in no apparent reduction in acidic sugar moieties. These findings strongly indicate that YlMpo1p performs a significant role in mannosylphosphorylation in Y. lipolytica with no involvement of the Mnn6p homologues. Mutant strains harboring the YlMPO1 gene disruption may serve as useful platforms for engineering Y. lipolytica glycosylation pathways for humanized glycans without any yeast-specific acidic modifications.-
dc.publisherAmer Soc Microb-
dc.titleEssential role of YlMPO1, a novel Yarrowia lipolytica homologue of Saccharomyces cerevisiae MNN4, in mannosylphosphorylation of N- and O-linked glycans-
dc.title.alternativeEssential role of YlMPO1, a novel Yarrowia lipolytica homologue of Saccharomyces cerevisiae MNN4, in mannosylphosphorylation of N- and O-linked glycans-
dc.typeArticle-
dc.citation.titleApplied and Environmental Microbiology-
dc.citation.number4-
dc.citation.endPage1195-
dc.citation.startPage1187-
dc.citation.volume77-
dc.contributor.affiliatedAuthorOh Suk Kwon-
dc.contributor.affiliatedAuthorDoo-Byoung Oh-
dc.contributor.alternativeName박정남-
dc.contributor.alternativeName송윤경-
dc.contributor.alternativeName천선아-
dc.contributor.alternativeName권오석-
dc.contributor.alternativeName오두병-
dc.contributor.alternativeNameJigami-
dc.contributor.alternativeName김정윤-
dc.contributor.alternativeName강현아-
dc.identifier.bibliographicCitationApplied and Environmental Microbiology, vol. 77, no. 4, pp. 1187-1195-
dc.identifier.doi10.1128/AEM.02323-10-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Aging Convergence Research Center > 1. Journal Articles
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