Aberrant up-regulation of LAMB3 and LAMC2 by promoter demethylation in gastric cancer

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Title
Aberrant up-regulation of LAMB3 and LAMC2 by promoter demethylation in gastric cancer
Author(s)
Oh Hyung Kwon; Jong Lyul ParkMirang KimJeong Hwan Kim; H C Lee; Hee-Jin Kim; S M Noh; K S Song; Hyang Sook Yoo; S G Paik; Seon-Young Kim; Yong Sung Kim
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 406, no. 4, pp. 539-545
Publication Year
2011
Abstract
The LAMB3 and LAMC2 genes encode the laminin-5 β3 and γ2 chains, respectively, which are parts of laminin-5, one of the major components of the basement membrane zone. Here, we report the frequent up-regulation of LAMB3 and LAMC2 by promoter demethylation in gastric cancer. Gene expression data analysis showed that LAMB3 and LAMC2 were up-regulated in various tumor tissues. Combined analyses of DNA methylation and gene expression of both genes in gastric cancer cell lines and tissues showed that DNA hypomethylation was associated with the up-regulation of both genes. Treatment with a methylation inhibitor induced LAMB3 and LAMC2 expression in gastric cancer cell lines in which both genes were silenced. By chromatin immunoprecipitation assay, we showed the activation histone mark H3K4me3 was associated with the expression of both genes. The expression level of LAMB3 affected multiple malignant phenotypes in gastric cancer cell lines. These results suggest that epigenetic activation of LAMB3 and LAMC2 may play an important role in gastric carcinogenesis.
Keyword
Gastric carcinogenesisMalignant phenotypeDemethylation
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2011.02.082
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
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