Bioinformatic analysis and annotation of expressed sequence tags (ESTs) generated from Anopheles sinensis mosquitoes challenged with apoptosis-inducing chemical, actinomycin-D

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Title
Bioinformatic analysis and annotation of expressed sequence tags (ESTs) generated from Anopheles sinensis mosquitoes challenged with apoptosis-inducing chemical, actinomycin-D
Author(s)
Y H Jo; Y S Lee; Se Won Kang; W G Kho; Hong-Seog Park; Sang-Haeng Choi; Y J Kim; Y S Hong; M Y Noh; S H Oh; I Kim; Y S Han
Bibliographic Citation
Entomological Research, vol. 41, no. 2, pp. 53-59
Publication Year
2011
Abstract
In 1993, after 10 years of absence, malaria re-emerged in the Republic of Korea in Gyeonggi and Gangwon Provinces near the Demilitarized Zone (DMZ). Anopheline mosquitoes are known to play a critical role in malaria transmission. Several studies have used gene expression data to examine the transcription responses of Anopheles gambiae after microbial and Plasmodium infections; however, as of now, there have been no reports of studies concerning the innate immune response and apoptosis in Anopheles sinensis. We have constructed two independent cDNA libraries from An. sinensis challenged with actinomycin-D (T, treated with apoptosis-inducing chemical) and without actinomycin-D (C, control). Analysis of the 5704 (C, 2848; T, 2856) expression sequence tags (ESTs) generated from these libraries identified 884 contigs (C, 421; T, 463) and 2180 singletons (C, 1038; T, 1142) with average lengths of 831 and 817bp, respectively. Furthermore, comparison of our EST sequences against the eukaryotic orthologous group database showed that 4324 (75.82%) of 5704 sequences could be grouped into three major functional categories. These EST data provide the basis for further studies characterizing the molecular mechanisms of midgut apoptosis.
Keyword
Anopheles sinensisApoptosisExpressed sequence tags (ESTs)KoreaMalaria
ISSN
1738-2297
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1748-5967.2011.00319.x
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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