An improved method for Agrobacterium-mediated genetic transformation from cotyledon explants of Brassica juncea

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dc.contributor.authorM S U Bhuiyan-
dc.contributor.authorSung Ran Min-
dc.contributor.authorWon Joong Jeong-
dc.contributor.authorS Sultana-
dc.contributor.authorK S Choi-
dc.contributor.authorY P Lim-
dc.contributor.authorW Y Song-
dc.contributor.authorY Lee-
dc.contributor.authorJang Ryol Liu-
dc.date.accessioned2017-04-19T09:22:18Z-
dc.date.available2017-04-19T09:22:18Z-
dc.date.issued2011-
dc.identifier.issn1342-4580-
dc.identifier.uri10.5511/plantbiotechnology.10.0921ako
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10085-
dc.description.abstractAn efficient Agrobacterium-mediated genetic transformation method was established for Brassica juncea by investigating several factors responsible for successful gene transfer. Four-day-old cotyledon explants from in vitro grown seedlings were co-cultivated with Agrobacterium strain GV3101 harboring the binary vector EnPCAMBIA1302-YCF1, which contained the hygromycin phosphotransferase (HPT) gene as a selectable marker and the yeast cadmium factor 1 (YCF1) gene. Two days co-cultivation period on shoot induction medium (MS medium supplemented with 0.1mgl -1 α- naphthaleneacetic acid, 1.0mgl 1 6-benzyladenine, and 2.0mgl -1 silver nitrate) containing 20mgl -1 acetosyringone and five days delaying exposure of explants to selective agent enhanced transformation efficiency significantly. A three-step selection strategy was developed to select hygromycin resistant shoots. Hygromycin-resistant shoots were subsequently rooted on root induction medium. Rooted plantlets were transferred to pot-soil, hardened, and grown in a greenhouse until maturity. Using the optimized transformation procedure, transformation efficiency reached at 16.2% in this study. Southern blot analysis was performed to confirm that transgenes (HPT and YCF1) were stably integrated into the plant genome. All transgenic plants showed single-copy of transgene integration in the host genome. Segregation analysis of T1 progeny showed that the transgenes were stably integrated and transmitted to the progeny in a Mendelian fashion.-
dc.publisherJapanese Soc Plant Cell & Molecular Biology-
dc.titleAn improved method for Agrobacterium-mediated genetic transformation from cotyledon explants of Brassica juncea-
dc.title.alternativeAn improved method for Agrobacterium-mediated genetic transformation from cotyledon explants of Brassica juncea-
dc.typeArticle-
dc.citation.titlePlant Biotechnology-
dc.citation.number1-
dc.citation.endPage23-
dc.citation.startPage17-
dc.citation.volume28-
dc.contributor.affiliatedAuthorSung Ran Min-
dc.contributor.affiliatedAuthorWon Joong Jeong-
dc.contributor.affiliatedAuthorJang Ryol Liu-
dc.contributor.alternativeNameBhuiyan-
dc.contributor.alternativeName민성란-
dc.contributor.alternativeName정원중-
dc.contributor.alternativeNameSultana-
dc.contributor.alternativeName최관삼-
dc.contributor.alternativeName임용표-
dc.contributor.alternativeName송원용-
dc.contributor.alternativeName이영숙-
dc.contributor.alternativeName유장렬-
dc.identifier.bibliographicCitationPlant Biotechnology, vol. 28, no. 1, pp. 17-23-
dc.identifier.doi10.5511/plantbiotechnology.10.0921a-
dc.subject.keywordCo-cultivation-
dc.subject.keywordCotyledon explants-
dc.subject.keywordGenetic transformation-
dc.subject.keywordHygromycin-
dc.subject.keywordIndian mustard-
dc.subject.localCo-cultivation-
dc.subject.localCotyledon explants-
dc.subject.localGenetic transformation-
dc.subject.localgenetic transformation-
dc.subject.localHygromycin-
dc.subject.localIndian mustard-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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