Production of stable chloroplast-transformed plants in potato (Solanum tuberosum L.) = 안정적 감자 엽록체 형질전환 식물체 생산

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dc.contributor.authorSung Ran Min-
dc.contributor.authorWon Joong Jeong-
dc.contributor.authorJi Hyun Park-
dc.contributor.authorJae Il Lyu-
dc.contributor.authorJeong Hee Lee-
dc.contributor.authorKwang Hoon Oh-
dc.contributor.authorH J Chung-
dc.contributor.authorJang Ryol Liu-
dc.date.accessioned2017-04-19T09:22:25Z-
dc.date.available2017-04-19T09:22:25Z-
dc.date.issued2011-
dc.identifier.issn1229-2818-
dc.identifier.uri10.5010/JPB.2011.38.1.042ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10089-
dc.description.abstractChloroplast genetic engineering of higher plants offers several unique advantages compared with nuclear genome transformation, such as high levels of transgene expression, a lack of position effect due to site-specific transgene integration by homologous recombination, multigene engineering in a single transformation event and reducing risks of gene flow via pollen due to maternal inheritance. We established a reproducible chloroplast transformation system of potato using a tobacco specific plastid transformation vector, pCtVG (trnI-Prrn-aadA-mgfp-TpsbA-trnA). Stable transgene integration into chloroplast genomes and the homoplasmic state of the transgenome were confirmed by PCR and Southern blot analyses. Northern, immunoblot analysis, and GFP fluorescence imaging revealed high expression and accumulation of GFP in the plastids of potato leaves. This system would provide new opportunities for genetic improvement and mass production of value added foreign proteins in this crop.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleProduction of stable chloroplast-transformed plants in potato (Solanum tuberosum L.) = 안정적 감자 엽록체 형질전환 식물체 생산-
dc.title.alternativeProduction of stable chloroplast-transformed plants in potato (Solanum tuberosum L.)-
dc.typeArticle-
dc.citation.titleJournal of Plant Biotechnology-
dc.citation.number1-
dc.citation.endPage48-
dc.citation.startPage42-
dc.citation.volume38-
dc.contributor.affiliatedAuthorSung Ran Min-
dc.contributor.affiliatedAuthorWon Joong Jeong-
dc.contributor.affiliatedAuthorJi Hyun Park-
dc.contributor.affiliatedAuthorJae Il Lyu-
dc.contributor.affiliatedAuthorJeong Hee Lee-
dc.contributor.affiliatedAuthorKwang Hoon Oh-
dc.contributor.affiliatedAuthorJang Ryol Liu-
dc.contributor.alternativeName민성란-
dc.contributor.alternativeName정원중-
dc.contributor.alternativeName박지현-
dc.contributor.alternativeName유재일-
dc.contributor.alternativeName이정희-
dc.contributor.alternativeName오광훈-
dc.contributor.alternativeName정화지-
dc.contributor.alternativeName유장렬-
dc.identifier.bibliographicCitationJournal of Plant Biotechnology, vol. 38, no. 1, pp. 42-48-
dc.identifier.doi10.5010/JPB.2011.38.1.042-
dc.description.journalClassN-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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