Novel quantitative method for the degree of branching in dextran

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dc.contributor.authorYoung Min Kim-
dc.contributor.authorA Kimura-
dc.contributor.authorD Kim-
dc.date.accessioned2017-04-19T09:22:48Z-
dc.date.available2017-04-19T09:22:48Z-
dc.date.issued2011-
dc.identifier.issn1226-7708-
dc.identifier.uri10.1007/s10068-011-0075-9ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10107-
dc.description.abstractA novel quantitative method for the determination of degree of branching in Leuconostoc mesenteroides B- 512F dextran was developed by using the combination of 3 dextran-degrading enzymes. First, Paenibacillus sp. endo-dextranase was randomly degraded B-512F dextran into linear or branched isomalto-oligosaccharides with various degree of polymerization (2-8). Second, Streptococcus mutans dextran glucosidase hydrolyzed linear or branched isomalto-oligosaccharides into glucose and branched isomalto-penta-saccharides. Third, the branched isomaltopenta- saccharide was degraded into glucose by using Bacteroides thetaimicron α-glucosidase. The number of branching points in B-512F dextran (5.42%) was determined by the difference in the amount of glucose in the reaction digest between BTGase-PDex and DGase-PDex treatments.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleNovel quantitative method for the degree of branching in dextran-
dc.title.alternativeNovel quantitative method for the degree of branching in dextran-
dc.typeArticle-
dc.citation.titleFood Science and Biotechnology-
dc.citation.number2-
dc.citation.endPage541-
dc.citation.startPage537-
dc.citation.volume20-
dc.contributor.affiliatedAuthorYoung Min Kim-
dc.contributor.alternativeName김영민-
dc.contributor.alternativeNameKimura-
dc.contributor.alternativeName김도만-
dc.identifier.bibliographicCitationFood Science and Biotechnology, vol. 20, no. 2, pp. 537-541-
dc.identifier.doi10.1007/s10068-011-0075-9-
dc.subject.keywordDegree of branching-
dc.subject.keywordDextran-
dc.subject.keywordEndo-dextranase-
dc.subject.keywordExo-dextranase-
dc.subject.localDegree of branching-
dc.subject.localDextran-
dc.subject.localdextran-
dc.subject.localEndo-dextranase-
dc.subject.localendo-dextranase-
dc.subject.localExo-dextranase-
dc.description.journalClassY-
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