DC Field | Value | Language |
---|---|---|
dc.contributor.author | H J Ryu | - |
dc.contributor.author | E S Seo | - |
dc.contributor.author | H K Kang | - |
dc.contributor.author | Young Min Kim | - |
dc.contributor.author | D Kim | - |
dc.date.accessioned | 2017-04-19T09:22:48Z | - |
dc.date.available | 2017-04-19T09:22:48Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1226-7708 | - |
dc.identifier.uri | 10.1007/s10068-011-0079-5 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10108 | - |
dc.description.abstract | A gene encoding human intestinal maltase (HMA) was successfully expressed in Pichia pastoris under the control of the methanol-induced alcohol oxidase (AOX1) promoter. The secreted recombinant HMA fused with a His6-tag was produced (150 U/L) and was easily purified from culture supernatants in a 3-step diafiltration, ultrafiltration, and affinity column chromatography protocol. The specific activity of the purified HMA was 16.8 U/mg. Endoglycosidase H digestion of the protein showed that the recombinant HMA was N-glycosylated. The purified HMA was maximally active at pH 6.5 and stable (≥90%) up to 65°C. The kinetic parameters Km and Vmax were 3.3±0.25 mM maltose and 61.9±2 U/mg, respectively. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Expression, purification, and characterization of human intestinal maltase secreted from Pichia pastoris | - |
dc.title.alternative | Expression, purification, and characterization of human intestinal maltase secreted from Pichia pastoris | - |
dc.type | Article | - |
dc.citation.title | Food Science and Biotechnology | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 565 | - |
dc.citation.startPage | 561 | - |
dc.citation.volume | 20 | - |
dc.contributor.affiliatedAuthor | Young Min Kim | - |
dc.contributor.alternativeName | 류화자 | - |
dc.contributor.alternativeName | 서은성 | - |
dc.contributor.alternativeName | 강희경 | - |
dc.contributor.alternativeName | 김영민 | - |
dc.contributor.alternativeName | 김도만 | - |
dc.identifier.bibliographicCitation | Food Science and Biotechnology, vol. 20, no. 2, pp. 561-565 | - |
dc.identifier.doi | 10.1007/s10068-011-0079-5 | - |
dc.subject.keyword | α-glucosidase | - |
dc.subject.keyword | Diabetes | - |
dc.subject.keyword | Expression in yeast | - |
dc.subject.keyword | Maltase | - |
dc.subject.keyword | Pichia pastoris | - |
dc.subject.local | α-glucosidase | - |
dc.subject.local | α-Glucosidase | - |
dc.subject.local | Alpha-glucosidase | - |
dc.subject.local | alpha-glucosidases | - |
dc.subject.local | Diabetes | - |
dc.subject.local | diabetes | - |
dc.subject.local | Expression in yeast | - |
dc.subject.local | Maltase | - |
dc.subject.local | pichia pastoris | - |
dc.subject.local | Pichia pastoris | - |
dc.description.journalClass | Y | - |
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