Expression, purification, and characterization of human intestinal maltase secreted from Pichia pastoris

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dc.contributor.authorH J Ryu-
dc.contributor.authorE S Seo-
dc.contributor.authorH K Kang-
dc.contributor.authorYoung Min Kim-
dc.contributor.authorD Kim-
dc.date.accessioned2017-04-19T09:22:48Z-
dc.date.available2017-04-19T09:22:48Z-
dc.date.issued2011-
dc.identifier.issn1226-7708-
dc.identifier.uri10.1007/s10068-011-0079-5ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10108-
dc.description.abstractA gene encoding human intestinal maltase (HMA) was successfully expressed in Pichia pastoris under the control of the methanol-induced alcohol oxidase (AOX1) promoter. The secreted recombinant HMA fused with a His6-tag was produced (150 U/L) and was easily purified from culture supernatants in a 3-step diafiltration, ultrafiltration, and affinity column chromatography protocol. The specific activity of the purified HMA was 16.8 U/mg. Endoglycosidase H digestion of the protein showed that the recombinant HMA was N-glycosylated. The purified HMA was maximally active at pH 6.5 and stable (≥90%) up to 65°C. The kinetic parameters Km and Vmax were 3.3±0.25 mM maltose and 61.9±2 U/mg, respectively.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleExpression, purification, and characterization of human intestinal maltase secreted from Pichia pastoris-
dc.title.alternativeExpression, purification, and characterization of human intestinal maltase secreted from Pichia pastoris-
dc.typeArticle-
dc.citation.titleFood Science and Biotechnology-
dc.citation.number2-
dc.citation.endPage565-
dc.citation.startPage561-
dc.citation.volume20-
dc.contributor.affiliatedAuthorYoung Min Kim-
dc.contributor.alternativeName류화자-
dc.contributor.alternativeName서은성-
dc.contributor.alternativeName강희경-
dc.contributor.alternativeName김영민-
dc.contributor.alternativeName김도만-
dc.identifier.bibliographicCitationFood Science and Biotechnology, vol. 20, no. 2, pp. 561-565-
dc.identifier.doi10.1007/s10068-011-0079-5-
dc.subject.keywordα-glucosidase-
dc.subject.keywordDiabetes-
dc.subject.keywordExpression in yeast-
dc.subject.keywordMaltase-
dc.subject.keywordPichia pastoris-
dc.subject.localα-glucosidase-
dc.subject.localα-Glucosidase-
dc.subject.localAlpha-glucosidase-
dc.subject.localalpha-glucosidases-
dc.subject.localDiabetes-
dc.subject.localdiabetes-
dc.subject.localExpression in yeast-
dc.subject.localMaltase-
dc.subject.localpichia pastoris-
dc.subject.localPichia pastoris-
dc.description.journalClassY-
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