Effect of constitutively active ras overexpression on cell growth in recombinant chinese hamster ovary cells

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Title
Effect of constitutively active ras overexpression on cell growth in recombinant chinese hamster ovary cells
Author(s)
Yeon Gu Kim; Y K Han; J Y Kim; Eun Gyo LeeHong-Weon Lee; G M Lee
Bibliographic Citation
Biotechnology Progress, vol. 27, no. 2, pp. 577-580
Publication Year
2011
Abstract
Constitutively active Ras (CA-Ras) is known to enhance cell growth through the induction of various signaling cascades including the phosphoinositide 3-kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK)/ERK signaling pathways, although the cellular response is highly dependent on the cell type. To evaluate the effect of CA-Ras overexpression on cell growth in recombinant Chinese hamster ovary (rCHO) cells, an erythropoietin (EPO)-producing rCHO cell line with regulated CA-Ras overexpression (EPO-off-CA-Ras) was established using the Tet-off system. The CA-Ras expression level in EPO-off-CA-Ras cells was tightly regulated by doxycycline addition. Although CA-Ras overexpression slightly increased the viable cell concentration during the late exponential phase, it did not increase the maximum viable cell concentration or specific growth rate to a significant degree. Unexpectedly, CA-Ras overexpression in rCHO cells led only to the enhancement in the activation of the MAPK/ERK signaling pathway and not the PI3K/Akt signaling pathway. Taken together, CA-Ras overexpression in rCHO cells did not significantly affect cell growth; it also had no critical impact on viable cell concentration or EPO production, possibly due to a failure to activate the PI3K/Akt signaling pathway.
Keyword
Cell growthErythropoietinInducible expressionRasRCHO cells
ISSN
8756-7938
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/btpr.567
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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