Oxygen tension regulates NK cells differentiation from hematopoietic stem cells in vitro

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Title
Oxygen tension regulates NK cells differentiation from hematopoietic stem cells in vitro
Author(s)
So Hyun Yoon; S H Lee; Suk Ran Yoon; P K Myung; In Pyo Choi
Bibliographic Citation
Immunology Letters, vol. 137, no. 1, pp. 70-77
Publication Year
2011
Abstract
Natural killer (NK) cells are differentiated from hematopoietic stem cells (HSCs) which are located at the lowest end of an oxygen gradient within the bone marrow (BM). In this report, we investigated whether oxygen tension could affect NK cell differentiation from hematopoietic cells in vitro. We found that hypoxia led to an inhibition of differentiation in NK cells, and increased oxygen supply alleviated this inhibition and restored NK cell differentiation under hypoxic condition. Hypoxia-treated cells demonstrated reduced mRNA expression of transcription factors (TFs) that have important roles in NK cell differentiation, such as EOMES, T-bet, GATA-3 and ETS-1. Moreover, hypoxia-pretreated cells recovered mRNA expression of TFs when the oxygen tension was changed to normoxia. Our findings suggest that oxygen tension modulates in vitro differentiation of NK cells through the regulation of TF expression.
Keyword
DifferentiationHematopoietic stem cellsHypoxiaNK cellsTranscription factors
ISSN
0165-2478
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.imlet.2011.02.020
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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