DC Field | Value | Language |
---|---|---|
dc.contributor.author | H R Jin | - |
dc.contributor.author | X Jin | - |
dc.contributor.author | N T Dat | - |
dc.contributor.author | Jung Joon Lee | - |
dc.date.accessioned | 2017-04-19T09:22:55Z | - |
dc.date.available | 2017-04-19T09:22:55Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | 10.1002/jcb.23078 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10117 | - |
dc.description.abstract | Cucurbitacin B, a natural triterpenoid is well-known for its strong anticancer activity, and recent studies showed that the compound inhibits JAK/STAT3 pathway. In this study, we demonstrate for the first time that cucurbitacin B is also a potent inhibitor of NF-κB activation. Our results showed that cucurbitacin B inhibited TNF-α-induced expression of NF-κB reporter gene and NF-κB target genes in a dose-dependent manner, however, it did not prevent either stimuli-induced degradation of IκBα or nuclear translocation and DNA-binding activity of NF-κB. On the other hand, cucurbitacin B dose-dependently suppressed not only NF-κB activation induced by overexpression of RelA/p65 but also transactivation activity of RelA/p65 subunit of NF-κB. Consistently, treatment of HeLa cells with the compound significantly suppressed TNF-α-induced activation of Akt and phosphorylation of Ser536 in RelA/p65, which is required for transactivation activity. Consequently, cucurbitacin B inhibited TNF-α-induced expression of NF-κB-dependent anti-apoptotic proteins such as c-IAP1, c-IAP2, XIAP, TRAF1, and TRAF2 and sensitized TNF-α-induced cell death. Taken together, our results demonstrated that cucurbitacin B could be served as a valuable candidate for the intervention of NF-κB-dependent pathological condition such as cancer. | - |
dc.publisher | Wiley | - |
dc.title | Cucurbitacin B suppresses the transactivation activity of RelA/p65 | - |
dc.title.alternative | Cucurbitacin B suppresses the transactivation activity of RelA/p65 | - |
dc.type | Article | - |
dc.citation.title | Journal of Cellular Biochemistry | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 1650 | - |
dc.citation.startPage | 1643 | - |
dc.citation.volume | 112 | - |
dc.contributor.affiliatedAuthor | Jung Joon Lee | - |
dc.contributor.alternativeName | Jin | - |
dc.contributor.alternativeName | Jin | - |
dc.contributor.alternativeName | Dat | - |
dc.contributor.alternativeName | 이정준 | - |
dc.identifier.bibliographicCitation | Journal of Cellular Biochemistry, vol. 112, no. 6, pp. 1643-1650 | - |
dc.identifier.doi | 10.1002/jcb.23078 | - |
dc.subject.keyword | AKT | - |
dc.subject.keyword | anti-apoptosis | - |
dc.subject.keyword | cucurbitacin B | - |
dc.subject.keyword | NF-κB | - |
dc.subject.keyword | RELA/p65 | - |
dc.subject.keyword | transactivation | - |
dc.subject.local | AKT | - |
dc.subject.local | Akt | - |
dc.subject.local | anti-apoptosis | - |
dc.subject.local | antiapoptosis | - |
dc.subject.local | Anti-apoptosis | - |
dc.subject.local | cucurbitacin B | - |
dc.subject.local | Nuclear factor-kappa B | - |
dc.subject.local | nuclear factor κB | - |
dc.subject.local | Nf-κb | - |
dc.subject.local | NF-kB | - |
dc.subject.local | nuclear factor kappa B | - |
dc.subject.local | NF-κB (nuclear factor kappa-B) | - |
dc.subject.local | NF-kappaB | - |
dc.subject.local | Nuclear factor-κb | - |
dc.subject.local | NF-κ B | - |
dc.subject.local | NF-κB | - |
dc.subject.local | NF-kappa B | - |
dc.subject.local | Nuclear factor κB (NF-κB) | - |
dc.subject.local | Nuclear factor κB | - |
dc.subject.local | NFκB | - |
dc.subject.local | Nf-κB | - |
dc.subject.local | Nuclear factor-κB | - |
dc.subject.local | nuclear factorκB | - |
dc.subject.local | Nuclear factor (NF)-κB | - |
dc.subject.local | Nuclear factor kappa B | - |
dc.subject.local | nuclear factor-κB | - |
dc.subject.local | NF-ΚB | - |
dc.subject.local | Nuclear factor-kappa B (NF-κB) | - |
dc.subject.local | Nuclear factor-kappaB | - |
dc.subject.local | nuclear factor-kappaB | - |
dc.subject.local | nuclear factor-kappaB (NF-κB) | - |
dc.subject.local | NFkappaB | - |
dc.subject.local | Nuclear factor kappaB | - |
dc.subject.local | RELA/p65 | - |
dc.subject.local | RelA/p65 | - |
dc.subject.local | Transactivation | - |
dc.subject.local | transactivation | - |
dc.description.journalClass | Y | - |
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