Vagococcus acidifermentans sp. nov., isolated from an acidogenic fermentation bioreactor

Abstract

Gram-staining-positive, coccus-shaped, non-spore-forming, facultatively anaerobic bacterium, designated AC-1T, was isolated from an acidogenic fermentation bioreactor treating food wastewater. On the basis of 16S rRNA gene sequence analysis, strain AC-1T was shown to belong to the genus Vagococcus. The closest phylogenetic relatives were Vagococcus elongatus PPC9T (97.4% 16S rRNA gene sequence similarity), Vagococcus penaei CD276T (96.7%) and Vagococcus carniphilus ATCC BAA-640T (96.6%). The major fatty acids were C18: 1ω9c (24.8%) and C16: 0(19.5%) and the G+C content of genomic DNA was 44.2 mol%, which supported the affiliation of strain AC-1T to the genus Vagococcus. Strain AC-1T and V. elongates DSM 21480T exhibited 11% DNA-DNA relatedness. Physiological and biochemical tests differentiated strain AC-1T from the type strains of recognized species of the genus Vagococcus. Therefore, strain AC-1T is considered to represent a novel species, for which the name Vagococcus acidifermentans sp. nov. is proposed. The type strain is AC-1T (=KCTC 13418T =LMG 24798T).