Expansion cooling in the matrix plume is under-recognized in MALDI mass spectrometry

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dc.contributor.authorY J Bae-
dc.contributor.authorJeong Hee Moon-
dc.contributor.authorM S Kim-
dc.date.accessioned2017-04-19T09:23:28Z-
dc.date.available2017-04-19T09:23:28Z-
dc.date.issued2011-
dc.identifier.issn1044-0305-
dc.identifier.uri10.1007/s13361-011-0115-yko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10144-
dc.description.abstractTime-of-flight (TOF) mass spectra for a peptide (Y6) were obtained by utilizing matrix-assisted infrared laser desorption ionization (IR-MALDI) with glycerol as the matrix and by ultraviolet MALDI with α-cyano-4-hydroxycinnamic acid (CHCA), sinapinic acid (SA), and 2,5-dihydroxybenzoic acid (DHB). Collisional activation during ion extraction and exothermicity in the gas-phase proton transfer were found to be unimportant as the driving forces for in-source (ISD) and post-source (PSD) decays, indicating that the thermal energy acquired during photo-ablation is responsible for their occurrence. The temperatures of [Y6∈+∈H] + in the 'early' and 'late' matrix plumes were estimated by the kinetic analysis of the ISD and PSD yields, respectively. The order of the temperatures was glycerol∈<∈DHB∈?∈SA∈<∈ CHCA in the early plume and glycerol∈<∈DHB∈<∈SA∈ <∈CHCA in the late plume. For each matrix, the temperature in the late plume was lower than in the early plume by 300-400 K, which was attributed to expansion cooling. The model (thermalization followed by expansion cooling) proposed to explain the occurrence of both rapid ISD and slow PSD is not only in sharp contrast with but also mutually exclusive with the prevailing explanation that the exothermicity in proton transfer and in-plume collisional activation are the driving forces for ion fragmentation in MALDI. The model also explains why MALDI is more successful for mass spectrometry of labile molecules than other desorption techniques that do not utilize a matrix. Factors affecting the plume temperature are also discussed.-
dc.publisherAmer Soc Mass Spectr-
dc.titleExpansion cooling in the matrix plume is under-recognized in MALDI mass spectrometry-
dc.title.alternativeExpansion cooling in the matrix plume is under-recognized in MALDI mass spectrometry-
dc.typeArticle-
dc.citation.titleJournal of American Society for Mass Spectrometry-
dc.citation.number6-
dc.citation.endPage1078-
dc.citation.startPage1070-
dc.citation.volume22-
dc.contributor.affiliatedAuthorJeong Hee Moon-
dc.contributor.alternativeName배용진-
dc.contributor.alternativeName문정희-
dc.contributor.alternativeName김명수-
dc.identifier.bibliographicCitationJournal of American Society for Mass Spectrometry, vol. 22, no. 6, pp. 1070-1078-
dc.identifier.doi10.1007/s13361-011-0115-y-
dc.subject.keywordDissociation efficiency-
dc.subject.keywordExpansion cooling-
dc.subject.keywordIn-source decay-
dc.subject.keywordMALDI-
dc.subject.keywordPeptide dissociation-
dc.subject.keywordPlume temperature-
dc.subject.keywordPost-source decay-
dc.subject.localDissociation efficiency-
dc.subject.localExpansion cooling-
dc.subject.localIn-source decay-
dc.subject.localMALDI-
dc.subject.localPeptide dissociation-
dc.subject.localPlume temperature-
dc.subject.localPost-source decay-
dc.description.journalClassY-
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Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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