Flavanones and rotenoids from the roots of Amorpha fruticosa L. that inhibit bacterial neuraminidase = 쪽제비싸리 뿌리로부터 분리한 플라보노이드와 로테노이드 화합물의 박테리아 뉴라미니데이즈 저해활성

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Title
Flavanones and rotenoids from the roots of Amorpha fruticosa L. that inhibit bacterial neuraminidase = 쪽제비싸리 뿌리로부터 분리한 플라보노이드와 로테노이드 화합물의 박테리아 뉴라미니데이즈 저해활성
Author(s)
Y S Kim; Young Bae Ryu; M J Curtis-Long; H J Yuk; J K Cho; J Y Kim; K D Kim; Woo Song Lee; K H Park
Bibliographic Citation
Food and Chemical Toxicology, vol. 49, no. 8, pp. 1849-1856
Publication Year
2011
Abstract
Neuraminidase is a proven target in anti-viral drug development. It also appears to be important for infection by certain pathogenic bacteria and has been implicated in biofilm formation. Based on activity-guided fractionation, the acetone extract of Amorpha fruticosa roots gave four flavanones 1-4 and three rotenoids 5-7 which were identified as amoradicin (1), amorisin (2), isoamoritin (3), amoricin (4), amorphigeni (5), dalbinol (6), and 6-ketodehydroamorphigenin (7), respectively. All isolated inhibitors showed strong neuraminidase inhibition with IC50s between 0.12 and 22.03μM. In particular, amorisin 2 exhibited 120nM IC50, which is 30-fold more potent than the positive control, quercetin. In addition, this is the first report detailing rotenoids (IC50=8.34-16.74μM) exhibiting neuraminidase inhibition. Kinetic analysis revealed that all inhibitors were noncompetitive. The most active neuraminidase inhibitors (2, 3, 5, 6) were proven to be present in the native root in high quantities by HPLC. Finally, at concentrations where no toxicity was observed, 3 and 6 inhibited Pseudomonas aeruginosa biofilm production. 29.7% and 21.0% inhibition respectively was observed at 25μ.
Keyword
Amorpha fruticosaBacterial neuraminidaseBiofilmFlavanoneRoteoid
ISSN
0278-6915
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.fct.2011.04.038
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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