Asperlin induces G2/M arrest through ROS generation and ATM pathway in human cervical carcinoma cells

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Title
Asperlin induces G2/M arrest through ROS generation and ATM pathway in human cervical carcinoma cells
Author(s)
L He; M H Nan; H C Oh; Y H Kim; JaeHyuik Jang; R L Erikson; Jong Seog AhnBo Yeon Kim
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 409, no. 3, pp. 489-493
Publication Year
2011
Abstract
We exploited the biological activity of an antibiotic agent asperlin isolated from Aspergillus nidulans against human cervical carcinoma cells. We found that asperlin dramatically increased reactive oxygen species (ROS) generation accompanied by a significant reduction in cell proliferation. Cleavage of caspase-3 and PARP and reduction of Bcl-2 could also be detected after asperlin treatment to the cells. An anti-oxidant N-acetyl-. l-cysteine (NAC), however, blocked all the apoptotic effects of asperlin. The involvement of oxidative stress in asperlin induced apoptosis could be supported by the findings that ROS- and DNA damage-associated G2/M phase arrest and ATM phosphorylation were increased by asperlin. In addition, expression and phosphorylation of cell cycle proteins as well as G2/M phase arrest in response to asperlin were significantly blocked by NAC or an ATM inhibitor KU-55933 pretreatment. Collectively, our study proved for the first time that asperlin could be developed as a potential anti-cancer therapeutics through ROS generation in HeLa cells.
Keyword
ROSAsperlinATM
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2011.05.032
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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