Production of lipids containing high levels of docosahexaenoic acid by a newly isolated microalga, Aurantiochytrium sp. KRS101 = 신규 Aurantiochytrium 속 미세조류에 의한 DHA 고함유 바이오오일의 생산

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Title
Production of lipids containing high levels of docosahexaenoic acid by a newly isolated microalga, Aurantiochytrium sp. KRS101 = 신규 Aurantiochytrium 속 미세조류에 의한 DHA 고함유 바이오오일의 생산
Author(s)
Won-Kyung Hong; D Rairakhwada; P S Seo; S Y Park; B K Hur; Chul Ho KimJeong-Woo Seo
Bibliographic Citation
Applied Biochemistry and Biotechnology, vol. 164, no. 8, pp. 1468-1480
Publication Year
2011
Abstract
In the present study, a novel oleaginous Thraustochytrid containing a high content of docosahexaenoic acid (DHA) was isolated from a mangrove ecosystem in Malaysia. The strain identified as an Aurantiochytrium sp. by 18S rRNA sequencing and named KRS101 used various carbon and nitrogen sources, indicating metabolic versatility. Optimal culture conditions, thus maximizing cell growth, and high levels of lipid and DHA production, were attained using glucose (60 g l-1) as carbon source, corn steep solid (10 g l-1) as nitrogen source, and sea salt (15 g l-1). The highest biomass, lipid, and DHA production of KRS101 upon fed-batch fermentation were 50.2 g l-1 (16.7 g l-1 day-1), 21.8 g l-1 (44% DCW), and 8.8 g l-1 (40% TFA), respectively. Similar values were obtained when a cheap substrate like molasses, rather than glucose, was used as the carbon source (DCW of 52.44 g l-1, lipid and DHA levels of 20.2 and 8.83 g l-1, respectively), indicating that production of microbial oils containing high levels of DHA can be produced economically when the novel strain is used.
Keyword
Aurantiochytrium sp.Docosahexaenoic acidHeterotrophic microalgaLipid
ISSN
0273-2289
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s12010-011-9227-x
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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