VaSpoU1 (SpoU gene) may be involved in organelle rRNA/tRNA modification in Viscum album

Cited 1 time in scopus
Metadata Downloads
VaSpoU1 (SpoU gene) may be involved in organelle rRNA/tRNA modification in Viscum album
Joon Woo Ahn; Suk Weon Kim; Jang Ryol Liu; Won Joong Jeong
Bibliographic Citation
Plant Biotechnology Reports, vol. 5, no. 3, pp. 289-295
Publication Year
The SpoU family of proteins catalyzes the methylation of transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs). We characterized a putative tRNA/rRNA methyltransferase, VaSpoU1 of the SpoU family, from Viscum album (mistletoe). VaSpoU1 and other plant SpoU1s exhibit motifs of the SpoU methylase domain that are conserved with bacterial and yeast SpoU methyltransferases. VaSpoU1 transcripts were detected in the leaves and stems of V. album. VaSpoU1-GFP fusion proteins localized to both chloroplasts and mitochondria in Arabidopsis protoplasts. Sequence analysis similarly predicted that the plant SpoU1 proteins would localize to chloroplasts and mitochondria. Interestingly, mitochondrial localization of VaSpoU1 was inhibited by the deletion of a putative N-terminal presequence in Arabidopsis protoplasts. Therefore, VaSpoU1 may be involved in tRNA and/or rRNA methylation in both chloroplasts and mitochondria.
AdoMet (S-adenosyl-L methionine)MethyltransferaseSpoU familySpoU methylase domainSubcellular localization
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.