Inactivated sendai-virus-mediated fusion improves early development of cloned bovine embryos by avoiding endoplasmic-reticulum-stress-associated apoptosis = 초기 복제수정란 생산효율을 증가시키는 비활성화된 바이러스 외막 사용에 의한 체세포융합 방법

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Title
Inactivated sendai-virus-mediated fusion improves early development of cloned bovine embryos by avoiding endoplasmic-reticulum-stress-associated apoptosis = 초기 복제수정란 생산효율을 증가시키는 비활성화된 바이러스 외막 사용에 의한 체세포융합 방법
Author(s)
Bong Seok SongJi Su KimSeung-Bin YoonKyu-Sun Lee; D B Koo; D S Lee; Y K Choo; Jae Won Huh; Sang Rae Lee; Sun-Uk Kim; Sang-Hyun Kim; H M Kim; Kyu Tae Chang
Bibliographic Citation
Reproduction Fertility and Development, vol. 23, no. 6, pp. 826-836
Publication Year
2011
Abstract
Somatic cell nuclear transfer (SCNT) is a powerful tool, not only for producing cloned animals, but also in revealing various early developmental events. However, relatively little is known regarding the biological events and underlying mechanism(s) directly associated with early development of SCNT embryos. Here, we show that production of high-quality bovine SCNT blastocysts is dependent on the method used for fusion and the associated reduction in endoplasmic reticulum (ER) stress. During fusion between the donor cell and the enucleated oocyte, electrofusion triggers spontaneous oocyte activation, accompanied by an increase in intracellular Ca2+ and improper nuclear remodelling. These events can be greatly reduced by the use of Sendai virus (SV)-mediated fusion. Moreover, SV-SCNT improves the blastulation rate and blastocyst quality, defined by the number and ratio of inner cell mass and trophectoderm cells in each blastocyst, in comparison with electrofusion- mediated SCNT (E-SCNT). Interestingly, expression of ER-stress-associated genes and blastomere apoptosis were significantly increased in E-SCNT embryos. These increases could be reversed by inhibition of ER stress or by using the SV-mediated fusion method. Collectively, these results indicate that SV-mediated fusion improves the developmental competence and quality of SCNT blastocysts, by reducing ER-stress-associated apoptosis.
Keyword
electrofusionsomatic cell nuclear transfer
ISSN
1031-3613
Publisher
CSIRO Publishing
DOI
http://dx.doi.org/10.1071/RD10194
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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