Human HOXA5 homeodomain enhances protein transduction and its application to vascular inflammation

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dc.contributor.authorJ Y Lee-
dc.contributor.authorKyoung Sook Park-
dc.contributor.authorE J Cho-
dc.contributor.authorH K Joo-
dc.contributor.authorS K Lee-
dc.contributor.authorS D Lee-
dc.contributor.authorJ B Park-
dc.contributor.authorS J Chang-
dc.contributor.authorB H Jeon-
dc.date.accessioned2017-04-19T09:24:30Z-
dc.date.available2017-04-19T09:24:30Z-
dc.date.issued2011-
dc.identifier.issn0006291X-
dc.identifier.uri10.1016/j.bbrc.2011.05.139ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10230-
dc.description.abstractCellular protein delivery is an emerging technique by which exogenous recombinant proteins are delivered into mammalian cells across the membrane. We have developed an Escherichia coli expression vector including human specific gene sequences for protein cellular delivery. The plasmid was generated by ligation the nucleotides 770-817 of the homeobox A5 mRNA sequence which was matched with protein transduction domain (PTD) of homeodomain protein A5 (HOXA5) into pET expression vector. The cellular uptake of HOXA5-PTD-EGFP was detected in 1. min and its transduction reached a maximum at 1. h within cell lysates. The cellular uptake of HOXA5-EGFP at 37. °C was greater than in 4. °C. For study for the functional role of human HOXA5-PTD, we purified HOXA5-APE1/Ref-1 and applied it on monocyte adhesion. Pretreatment with HOXA5-APE1/Ref-1 (100. nM) inhibited TNF-α-induced monocyte adhesion to endothelial cells, compared with HOXA5-EGFP. Taken together, our data suggested that human HOXA5-PTD vector provides a powerful research tools for uncovering cellular functions of proteins or for the generation of human PTD-containing proteins.-
dc.publisherElsevier-
dc.titleHuman HOXA5 homeodomain enhances protein transduction and its application to vascular inflammation-
dc.title.alternativeHuman HOXA5 homeodomain enhances protein transduction and its application to vascular inflammation-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number2-
dc.citation.endPage316-
dc.citation.startPage312-
dc.citation.volume410-
dc.contributor.alternativeName이지영-
dc.contributor.alternativeName박경숙-
dc.contributor.alternativeName조은정-
dc.contributor.alternativeName주희경-
dc.contributor.alternativeName이상기-
dc.contributor.alternativeName이상도-
dc.contributor.alternativeName박진봉-
dc.contributor.alternativeName장석종-
dc.contributor.alternativeName전병화-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 410, no. 2, pp. 312-316-
dc.identifier.doi10.1016/j.bbrc.2011.05.139-
dc.subject.keywordCellular transduction-
dc.subject.keywordHOXA5-APE1/Ref-1-
dc.subject.keywordHOXA5-EGFP-
dc.subject.keywordHuman homeodomain protein A5-
dc.subject.keywordMonocyte adhesion-
dc.subject.localCellular transduction-
dc.subject.localHOXA5-APE1/Ref-1-
dc.subject.localHOXA5-EGFP-
dc.subject.localHuman homeodomain protein A5-
dc.subject.localMonocyte adhesion-
dc.description.journalClassY-
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