Trascriptome profiling of kidney tissue from FGS/kist mice, the Korean animal model of focal segmental glomerulosclerosis = 국소성 분절성 사구체 신병증의 동물 모델 (FGS/kist 생쥐) 신 조직의 유전자 발현 양상
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- Trascriptome profiling of kidney tissue from FGS/kist mice, the Korean animal model of focal segmental glomerulosclerosis = 국소성 분절성 사구체 신병증의 동물 모델 (FGS/kist 생쥐) 신 조직의 유전자 발현 양상
- H G Kang; B S Lee; Chul Ho Lee; I S Ha; H I Cheong; Y Choi
- Bibliographic Citation
- Journal of Korean Society of Pediatric Nephrology, vol. 15, no. 1, pp. 38-48
- Publication Year
- Purpose : Focal segmental glomerulosclerosis (FSGS) is the most common glomerulopathy
causing pediatric renal failure. Since specific treatment targeting the etiology and
pathophysiology of primary FSGS is yet elusive, the authors explored the pathophysiology
of FSGS by transcriptome analysis of the disease using an animal model. Conclusion : This study confirmed that renal cell death, immune system activation with
subsequent fibrosis, and lipid metabolism-related early vasculopathy were involved in the
pathophysiology of FSGS. In addition, the relevance of methodology used in this study,
namely transcriptome profiling, and Korean animal model of FGS/kist was validated. Further
study would reveal novel pathophysiology of FSGS for new therapeutic targets.
Methods : FGS/kist strain, a mouse model of primary FSGS, and RFM/kist strain, as control
and the parent strain of FGS/kist, were used. Kidney tissues were harvested and isolated
renal cortex was used to extract mRNA, which was run on AB 1700 mouse microarray
chip after reverse transcription to get the transcriptome profile.
Results : Sixty two genes were differentially expressed in FGS/kist kidney tissue compared
to the control. Those genes were related to cell cycle/cell death, immune reaction, and
lipid metabolism/vasculopathy, and the key molecules of their networks were TNF, IL-6/
4, IFNγ, TP53, and PPARγ.
- Focal segmental glomerulosclerosisTranscriptomeLaboratory Animal Models
- Korea Soc-Assoc-Inst
- Appears in Collections:
- Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
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