DNA barcodes for two scale insect families, mealybugs (Hemiptera: Pseudococcidae) and armored scales (Hemiptera: Diaspididae)

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Title
DNA barcodes for two scale insect families, mealybugs (Hemiptera: Pseudococcidae) and armored scales (Hemiptera: Diaspididae)
Author(s)
Doo Sang Park; S J Suh; P D N Hebert; Hyun Woo Oh; K J Hong
Bibliographic Citation
Bulletin of Entomological Research, vol. 101, no. 4, pp. 429-434
Publication Year
2011
Abstract
Although DNA barcode coverage has grown rapidly for many insect orders, there are some groups, such as scale insects, where sequence recovery has been difficult. However, using a recently developed primer set, we recovered barcode records from 373 specimens, providing coverage for 75 species from 31 genera in two families. Overall success was >90% for mealybugs and >80% for armored scale species. The GA?C content was very low in most species, averaging just 16.3%. Sequence divergences (K2P) between congeneric species averaged 10.7%, while intra-specific divergences averaged 0.97%. However, the latter value was inflated by high intra-specific divergence in nine taxa, cases that may indicate species overlooked by current taxonomic treatments. Our study establishes the feasibility of developing a comprehensive barcode library for scale insects and indicates that its construction will both create an effective system for identifying scale insects and reveal taxonomic situations worthy of deeper analysis.
Keyword
armored scaleCOIDNA barcodingGAC contentmealybugNUMTs
ISSN
0007-4853
Publisher
Cambridge Univ Press
DOI
http://dx.doi.org/10.1017/S0007485310000714
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Bio Technology Innovation > Core Facility Management Center > 1. Journal Articles
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