Phenotypic and functional maturation of dendritic cells induced by polysaccharide isolated from Paecilomyces cicadae

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Title
Phenotypic and functional maturation of dendritic cells induced by polysaccharide isolated from Paecilomyces cicadae
Author(s)
H S Kim; J Y Kim; H S Ryu; B R Shin; Jong Soon Kang; H M Kim; Y O Kim; J T Hong; Y Kim; S B Han
Bibliographic Citation
Journal of Medicinal Food, vol. 14, no. 7, pp. 847-856
Publication Year
2011
Abstract
Paecilomyces cicadae Miquel Samson is the anamorph of Cordyceps cicadae Shing and is used in functional foods for the prevention and treatment of various diseases. In the present study, we examined the effects of P. cicadae polysaccharide (PCP) on dendritic cell (DC) maturation. Phenotypic maturation of DCs by PCP was confirmed by the elevated expressions of CD80, CD86, major histocompatibility complex (MHC)-I, and MHC-II molecules and functional maturation by increased expression of interleukin-12, interleukin-1β, and tumor necrosis factor-α, enhanced allogenic T cell stimulation, and decreased endocytosis. PCP induced the maturation of DCs from C3H/HeN and C57BL/6 mice but not from Toll-like receptor (tlr) 4-/- knockout mice and TLR4-mutated C3H/HeJ mice, which suggests that TLR4 is the membrane receptor for PCP. PCP increased the degradation of inhibitor of nuclear factor-κB (NF-κB) α/β, which enhanced the nuclear translocation of NF-κB p50/p65 and induced the phosphorylation of extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 mitogen-activated protein kinases, which are signaling molecules downstream of TLR4. These results indicate that PCP induces DC maturation through TLR4 signaling.
Keyword
dendritic cell maturationmitogen-activated protein kinasesnuclear factor-jBPaecilomyces cicadaeToll-like receptor 4
ISSN
1096-620X
Publisher
Mary Ann Liebert, Inc
DOI
http://dx.doi.org/10.1089/jmf.2011.1575
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
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