Overexpression of 2-cysteine peroxiredoxin enhances tolerance to methyl viologen-mediated oxidative stress and high temperature in potato plants = 2-Cys Prx 과발현 감자식물체의 산화스트레스 및 고온 스트레스 내성증가

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Title
Overexpression of 2-cysteine peroxiredoxin enhances tolerance to methyl viologen-mediated oxidative stress and high temperature in potato plants = 2-Cys Prx 과발현 감자식물체의 산화스트레스 및 고온 스트레스 내성증가
Author(s)
Myoung Duck Kim; Yun Hee Kim; Suk Yoon Kwon; B Y Jang; S Y Lee; D J Yun; J H Cho; Sang Soo Kwak; Haeng Soon Lee
Bibliographic Citation
Plant Physiology and Biochemistry, vol. 49, no. 8, pp. 891-897
Publication Year
2011
Abstract
Oxidative stress is one of the major causative factors for injury to plants exposed to environmental stresses. Plants have developed diverse defense mechanisms for scavenging oxidative stress-inducing molecules. The antioxidative enzyme 2-cysteine peroxiredoxin (2-Cys Prx) removes peroxides and protects the photosynthetic membrane from oxidative damage. In this study, transgenic potato (Solanum tuberosum L. cv. Atlantic) expressing At2-Cys Prx under control of the oxidative stress-inducible SWPA2 promoter or enhanced CaMV 35S promoter (referred to as SP and EP plants, respectively) was generated using Agrobacterium-mediated transformation. The transgenic plants were tested for tolerance to stress. Following treatment with 3 μM methyl viologen (MV), leaf discs from SP and EP plants showed approximately 33 and 15% less damage than non-transformed (NT) plants. When 300 μM MV was sprayed onto whole plants, the photosynthetic activity of SP plants decreased by 25%, whereas that of NT plants decreased by 60%. In addition, SP plants showed enhanced tolerance to high temperature at 42 °C. After treatment at high temperature, the photosynthetic activity of SP plants decreased by about 7% compared to plants grown at 25 °C, whereas it declined by 31% in NT plants. These results indicate that transgenic potato can efficiently regulate oxidative stress from various environmental stresses via overexpression of At2-Cys Prx under control of the stress-inducible SWPA2 promoter.
Keyword
2-Cys PrxHigh temperatureOxidative stressPhotosynthetic activitySolanum tuberosum
ISSN
0981-9428
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.plaphy.2011.04.001
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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