Overexpression of 2-cysteine peroxiredoxin enhances tolerance to methyl viologen-mediated oxidative stress and high temperature in potato plants = 2-Cys Prx 과발현 감자식물체의 산화스트레스 및 고온 스트레스 내성증가
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- Overexpression of 2-cysteine peroxiredoxin enhances tolerance to methyl viologen-mediated oxidative stress and high temperature in potato plants = 2-Cys Prx 과발현 감자식물체의 산화스트레스 및 고온 스트레스 내성증가
- Myoung Duck Kim; Yun Hee Kim; Suk Yoon Kwon; B Y Jang; S Y Lee; D J Yun; J H Cho; Sang Soo Kwak; Haeng Soon Lee
- Bibliographic Citation
- Plant Physiology and Biochemistry, vol. 49, no. 8, pp. 891-897
- Publication Year
- Oxidative stress is one of the major causative factors for injury to plants exposed to environmental stresses. Plants have developed diverse defense mechanisms for scavenging oxidative stress-inducing molecules. The antioxidative enzyme 2-cysteine peroxiredoxin (2-Cys Prx) removes peroxides and protects the photosynthetic membrane from oxidative damage. In this study, transgenic potato (Solanum tuberosum L. cv. Atlantic) expressing At2-Cys Prx under control of the oxidative stress-inducible SWPA2 promoter or enhanced CaMV 35S promoter (referred to as SP and EP plants, respectively) was generated using Agrobacterium-mediated transformation. The transgenic plants were tested for tolerance to stress. Following treatment with 3 μM methyl viologen (MV), leaf discs from SP and EP plants showed approximately 33 and 15% less damage than non-transformed (NT) plants. When 300 μM MV was sprayed onto whole plants, the photosynthetic activity of SP plants decreased by 25%, whereas that of NT plants decreased by 60%. In addition, SP plants showed enhanced tolerance to high temperature at 42 °C. After treatment at high temperature, the photosynthetic activity of SP plants decreased by about 7% compared to plants grown at 25 °C, whereas it declined by 31% in NT plants. These results indicate that transgenic potato can efficiently regulate oxidative stress from various environmental stresses via overexpression of At2-Cys Prx under control of the stress-inducible SWPA2 promoter.
- 2-Cys PrxHigh temperatureOxidative stressPhotosynthetic activitySolanum tuberosum
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- Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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