Label-free electrochemical detection of human alpha-thrombin in blood serum using ferrocene-coated gold nanoparticles

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dc.contributor.authorDohyoung Kwon-
dc.contributor.authorHyun Kyung Chung-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T09:24:46Z-
dc.date.available2017-04-19T09:24:46Z-
dc.date.issued2011-
dc.identifier.issn0956-5663-
dc.identifier.uri10.1016/j.bios.2011.06.040ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10267-
dc.description.abstractThis paper describes a novel approach to label-free electrochemical detection of human α-thrombin in human blood serum that utilizes ferrocene-coated gold nanoparticles (Fc-AuNPs). Human α-thrombin was specifically bound by the thiolated aptamers immobilized on the electrode. Positively charged Fc-AuNPs were electrostatically bound to the negatively charged aptamers. In principle, a high current peak should be observed in the absence of interactions between the aptamers and the human α-thrombin. This behavior indicates maximum adsorption of Fc-AuNPs by the negatively charged aptamers on the electrode surface. In contrast, when the thrombin-aptamer complex is formed, a low signal is expected because of the blocking capacities of the protein, which hinders the electrostatic binding of the Fc-AuNPs. The electrochemical signal, recorded by cyclic voltammetry and differential pulse voltammetry, indicates whether interactions between aptamers and proteins have occurred. There is a good correlation between the ferrocene oxidation peak intensity readings from our thrombin sensing system and the thrombin concentration, within the range of 1.2 μM-12 pM.-
dc.publisherElsevier-
dc.titleLabel-free electrochemical detection of human alpha-thrombin in blood serum using ferrocene-coated gold nanoparticles-
dc.title.alternativeLabel-free electrochemical detection of human alpha-thrombin in blood serum using ferrocene-coated gold nanoparticles-
dc.typeArticle-
dc.citation.titleBiosensors & Bioelectronics-
dc.citation.number1-
dc.citation.endPage458-
dc.citation.startPage454-
dc.citation.volume28-
dc.contributor.affiliatedAuthorDohyoung Kwon-
dc.contributor.affiliatedAuthorHyun Kyung Chung-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName권도형-
dc.contributor.alternativeName정현경-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationBiosensors & Bioelectronics, vol. 28, no. 1, pp. 454-458-
dc.identifier.doi10.1016/j.bios.2011.06.040-
dc.subject.keywordAptamer-
dc.subject.keywordAptasensor-
dc.subject.keywordElectrochemistry-
dc.subject.keywordGold nanoparticles-
dc.subject.keywordLabel-free-
dc.subject.keywordThrombin-
dc.subject.localaptamer-
dc.subject.localAptamers-
dc.subject.localAptamer-
dc.subject.localAptasensor-
dc.subject.localaptasensor-
dc.subject.localElectrochemistry-
dc.subject.localgold nanoparticle-
dc.subject.localGold nanoparticles-
dc.subject.localGold nanoparticle (AuNP)-
dc.subject.localGold nanoparticle-
dc.subject.locallabel-free-
dc.subject.localLabel-free-
dc.subject.localthrombin-
dc.subject.localThrombin-
dc.description.journalClassY-
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