DC Field | Value | Language |
---|---|---|
dc.contributor.author | J S Shin | - |
dc.contributor.author | Y S Noh | - |
dc.contributor.author | Y S Lee | - |
dc.contributor.author | Y W Cho | - |
dc.contributor.author | N I Baek | - |
dc.contributor.author | M S Choi | - |
dc.contributor.author | Tae Sook Jeong | - |
dc.contributor.author | E Kang | - |
dc.contributor.author | H G Chung | - |
dc.contributor.author | K T Lee | - |
dc.date.accessioned | 2017-04-19T09:24:47Z | - |
dc.date.available | 2017-04-19T09:24:47Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0007-1188 | - |
dc.identifier.uri | 10.1111/j.1476-5381.2011.01351.x | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10274 | - |
dc.description.abstract | BACKGROUND AND PURPOSE Brassica rapa species constitute one of the major sources of food. In the present study, we investigated the anti-inflammatory effects and the underlying molecular mechanism of arvelexin, isolated from B. rapa, on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and on a model of septic shock induced by LPS. EXPERIMENTAL APPROACH The expression of Inducible nitric oxide synthase (iNOS) and COX-2, TNF-α, IL-6 and IL-1β were determined by Western blot and/or RT-PCR respectively. To elucidate the underlying mechanism(s), activation of NF-κB activation and its pathways were investigated by electrophoretic mobility shift assay, reporter gene and Western blot assays. In addition, the in vivo anti-inflammatory effects of arvelexin were evaluated in endotoxaemia induced with LPS. KEY RESULTS Promoter assays for iNOS and COX-2 revealed that arvelexin inhibited LPS-induced NO and prostaglandin E2 production through the suppression of iNOS and COX-2 at the level of gene transcription. In addition, arvelexin inhibited NF-κB-dependent inflammatory responses by modulating a series of intracellular events of IκB kinase (IKK)-inhibitor κBα (IκBα)-NF-κB signalling. Moreover, arvelexin inhibited IKKβ-elicited NF-κB activation as well as iNOS and COX-2 expression. Serum levels of NO and inflammatory cytokines and mortality in mice challenged injected with LPS were significantly reduced by arvelexin. CONCLUSION AND IMPLICATIONS Arvelexin down-regulated inflammatory iNOS, COX-2, TNF-α, IL-6 and IL-1β gene expression in macrophages interfering with the activation of IKKβ and p38 mitogen-activated protein kinase, and thus, preventing NF-κB activation. | - |
dc.publisher | Wiley | - |
dc.title | Arvelexin from Brassica rapa suppresses NF-κB-regulated pro-inflammatory gene expression by inhibiting activation of IκB kinase | - |
dc.title.alternative | Arvelexin from Brassica rapa suppresses NF-κB-regulated pro-inflammatory gene expression by inhibiting activation of IκB kinase | - |
dc.type | Article | - |
dc.citation.title | British Journal of Pharmacology | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 158 | - |
dc.citation.startPage | 145 | - |
dc.citation.volume | 164 | - |
dc.contributor.affiliatedAuthor | Tae Sook Jeong | - |
dc.contributor.alternativeName | 신 | - |
dc.contributor.alternativeName | 노 | - |
dc.contributor.alternativeName | 이 | - |
dc.contributor.alternativeName | 조 | - |
dc.contributor.alternativeName | 백남인 | - |
dc.contributor.alternativeName | 최명숙 | - |
dc.contributor.alternativeName | 정태숙 | - |
dc.contributor.alternativeName | 강 | - |
dc.contributor.alternativeName | 정 | - |
dc.contributor.alternativeName | 이 | - |
dc.identifier.bibliographicCitation | British Journal of Pharmacology, vol. 164, no. 1, pp. 145-158 | - |
dc.identifier.doi | 10.1111/j.1476-5381.2011.01351.x | - |
dc.subject.keyword | arvelexin | - |
dc.subject.keyword | Brassica rapa | - |
dc.subject.keyword | cyclooxygenase-2 | - |
dc.subject.keyword | IKK | - |
dc.subject.keyword | inducible nitric oxide | - |
dc.subject.keyword | inflammation | - |
dc.subject.keyword | NF-κB | - |
dc.subject.keyword | paw oedema | - |
dc.subject.local | arvelexin | - |
dc.subject.local | Brassica rapa | - |
dc.subject.local | brassica rapa | - |
dc.subject.local | Cyclooxygenase 2 | - |
dc.subject.local | Cyclooxygenase-2 | - |
dc.subject.local | Cyclooxygenase-2 (COX-2) | - |
dc.subject.local | cyclooxygenase-2 | - |
dc.subject.local | IKK | - |
dc.subject.local | inducible nitric oxide | - |
dc.subject.local | Inflammation | - |
dc.subject.local | inflammation | - |
dc.subject.local | nflammation | - |
dc.subject.local | NFkappaB | - |
dc.subject.local | NFκB | - |
dc.subject.local | Nf-κB | - |
dc.subject.local | Nf-κb | - |
dc.subject.local | Nuclear factor (NF)-κB | - |
dc.subject.local | Nuclear factor kappa B | - |
dc.subject.local | Nuclear factor kappaB | - |
dc.subject.local | Nuclear factor κB | - |
dc.subject.local | Nuclear factor κB (NF-κB) | - |
dc.subject.local | Nuclear factor-kappa B | - |
dc.subject.local | Nuclear factor-kappa B (NF-κB) | - |
dc.subject.local | Nuclear factor-kappaB | - |
dc.subject.local | Nuclear factor-κB | - |
dc.subject.local | Nuclear factor-κb | - |
dc.subject.local | NF-kB | - |
dc.subject.local | NF-kappa B | - |
dc.subject.local | NF-kappaB | - |
dc.subject.local | NF-ΚB | - |
dc.subject.local | NF-κ B | - |
dc.subject.local | NF-κB | - |
dc.subject.local | NF-κB (nuclear factor kappa-B) | - |
dc.subject.local | nuclear factor kappa B | - |
dc.subject.local | nuclear factor κB | - |
dc.subject.local | nuclear factor-kappaB | - |
dc.subject.local | nuclear factor-kappaB (NF-κB) | - |
dc.subject.local | nuclear factor-κB | - |
dc.subject.local | nuclear factorκB | - |
dc.subject.local | paw oedema | - |
dc.description.journalClass | Y | - |
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