Silver-stained fibrin zymography : separation of proteases and activity detection using a single substrate-containing gel = Silver-stained fibrin zymography : 단일기질 함유 겔을 이용한 프로테아제의 활성 검증 및 분리

Cited 4 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorDong Min Chung-
dc.contributor.authorK E Kim-
dc.contributor.authorKeug Hyun Ahn-
dc.contributor.authorChan Sun Park-
dc.contributor.authorD H Kim-
dc.contributor.authorH B Koh-
dc.contributor.authorHyo Kon Chun-
dc.contributor.authorByung Dae Yoon-
dc.contributor.authorH J Kim-
dc.contributor.authorMin-Soo Kim-
dc.contributor.authorN S Choi-
dc.date.accessioned2017-04-19T09:24:52Z-
dc.date.available2017-04-19T09:24:52Z-
dc.date.issued2011-
dc.identifier.issn0141-5492-
dc.identifier.uri10.1007/s10529-011-0619-3ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10285-
dc.description.abstractA new zymogram method, silver-stained fibrin zymography, for separation of protease bands and activity detection using a single substrate gel, was developed. The method takes advantage of the nanoscale sensitivity of both zymography and silver staining. After SDS-PAGE in a gel containing fibrin, the gel was incubated in enzyme reaction buffer and the zymogram was silver-stained. Bands with protease activity were stained with silver in clear areas where the protein substrate had been degraded. The molecular sizes of proteases were accurately determined. Furthermore, proteases of high molecular weight were clearly and sharply resolved.-
dc.publisherSpringer-
dc.titleSilver-stained fibrin zymography : separation of proteases and activity detection using a single substrate-containing gel = Silver-stained fibrin zymography : 단일기질 함유 겔을 이용한 프로테아제의 활성 검증 및 분리-
dc.title.alternativeSilver-stained fibrin zymography : separation of proteases and activity detection using a single substrate-containing gel-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number8-
dc.citation.endPage1666-
dc.citation.startPage1663-
dc.citation.volume33-
dc.contributor.affiliatedAuthorDong Min Chung-
dc.contributor.affiliatedAuthorKeug Hyun Ahn-
dc.contributor.affiliatedAuthorChan Sun Park-
dc.contributor.affiliatedAuthorHyo Kon Chun-
dc.contributor.affiliatedAuthorByung Dae Yoon-
dc.contributor.affiliatedAuthorMin-Soo Kim-
dc.contributor.alternativeName정동민-
dc.contributor.alternativeName김기은-
dc.contributor.alternativeName안극현-
dc.contributor.alternativeName박찬선-
dc.contributor.alternativeName김동호-
dc.contributor.alternativeName고홍범-
dc.contributor.alternativeName전효곤-
dc.contributor.alternativeName윤병대-
dc.contributor.alternativeName김홍집-
dc.contributor.alternativeName김민수-
dc.contributor.alternativeName최낙식-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 33, no. 8, pp. 1663-1666-
dc.identifier.doi10.1007/s10529-011-0619-3-
dc.subject.keywordProtease-
dc.subject.keywordSilver staining-
dc.subject.keywordZymography-
dc.subject.localprotease-
dc.subject.localProtease-
dc.subject.localProteases-
dc.subject.localSilver staining-
dc.subject.localZymography-
dc.subject.localzymography-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.