DC Field | Value | Language |
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dc.contributor.author | Dong Min Chung | - |
dc.contributor.author | K E Kim | - |
dc.contributor.author | Keug Hyun Ahn | - |
dc.contributor.author | Chan Sun Park | - |
dc.contributor.author | D H Kim | - |
dc.contributor.author | H B Koh | - |
dc.contributor.author | Hyo Kon Chun | - |
dc.contributor.author | Byung Dae Yoon | - |
dc.contributor.author | H J Kim | - |
dc.contributor.author | Min-Soo Kim | - |
dc.contributor.author | N S Choi | - |
dc.date.accessioned | 2017-04-19T09:24:52Z | - |
dc.date.available | 2017-04-19T09:24:52Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | 10.1007/s10529-011-0619-3 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10285 | - |
dc.description.abstract | A new zymogram method, silver-stained fibrin zymography, for separation of protease bands and activity detection using a single substrate gel, was developed. The method takes advantage of the nanoscale sensitivity of both zymography and silver staining. After SDS-PAGE in a gel containing fibrin, the gel was incubated in enzyme reaction buffer and the zymogram was silver-stained. Bands with protease activity were stained with silver in clear areas where the protein substrate had been degraded. The molecular sizes of proteases were accurately determined. Furthermore, proteases of high molecular weight were clearly and sharply resolved. | - |
dc.publisher | Springer | - |
dc.title | Silver-stained fibrin zymography : separation of proteases and activity detection using a single substrate-containing gel = Silver-stained fibrin zymography : 단일기질 함유 겔을 이용한 프로테아제의 활성 검증 및 분리 | - |
dc.title.alternative | Silver-stained fibrin zymography : separation of proteases and activity detection using a single substrate-containing gel | - |
dc.type | Article | - |
dc.citation.title | Biotechnology Letters | - |
dc.citation.number | 8 | - |
dc.citation.endPage | 1666 | - |
dc.citation.startPage | 1663 | - |
dc.citation.volume | 33 | - |
dc.contributor.affiliatedAuthor | Dong Min Chung | - |
dc.contributor.affiliatedAuthor | Keug Hyun Ahn | - |
dc.contributor.affiliatedAuthor | Chan Sun Park | - |
dc.contributor.affiliatedAuthor | Hyo Kon Chun | - |
dc.contributor.affiliatedAuthor | Byung Dae Yoon | - |
dc.contributor.affiliatedAuthor | Min-Soo Kim | - |
dc.contributor.alternativeName | 정동민 | - |
dc.contributor.alternativeName | 김기은 | - |
dc.contributor.alternativeName | 안극현 | - |
dc.contributor.alternativeName | 박찬선 | - |
dc.contributor.alternativeName | 김동호 | - |
dc.contributor.alternativeName | 고홍범 | - |
dc.contributor.alternativeName | 전효곤 | - |
dc.contributor.alternativeName | 윤병대 | - |
dc.contributor.alternativeName | 김홍집 | - |
dc.contributor.alternativeName | 김민수 | - |
dc.contributor.alternativeName | 최낙식 | - |
dc.identifier.bibliographicCitation | Biotechnology Letters, vol. 33, no. 8, pp. 1663-1666 | - |
dc.identifier.doi | 10.1007/s10529-011-0619-3 | - |
dc.subject.keyword | Protease | - |
dc.subject.keyword | Silver staining | - |
dc.subject.keyword | Zymography | - |
dc.subject.local | protease | - |
dc.subject.local | Protease | - |
dc.subject.local | Proteases | - |
dc.subject.local | Silver staining | - |
dc.subject.local | Zymography | - |
dc.subject.local | zymography | - |
dc.description.journalClass | Y | - |
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