Quercetin glucoside profiling of fresh onion (Allium cepa) and aged black onion using HPLC-ESI/MS/MS = HPLC-ESI/MS/MS를 이용한 생양파와 흑양파의 퀘세틴 배당체 분석

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Title
Quercetin glucoside profiling of fresh onion (Allium cepa) and aged black onion using HPLC-ESI/MS/MS = HPLC-ESI/MS/MS를 이용한 생양파와 흑양파의 퀘세틴 배당체 분석
Author(s)
Dong Min Chung; S H Kwon; Y C Chung; Hyo Kon Chun
Bibliographic Citation
Korean Journal of Life Sciences, vol. 21, no. 3, pp. 464-467
Publication Year
2011
Abstract
Quercetin is a major flavonoid present in onions, which acts as an antioxidant. Quercetin exists both as a free compound and conjugated with carbohydrates, primarily as glucosides in onion. Aged black onion was made through a 30 day aging process in which the onions were kept in an environment of 60oC and high humidity (90% RH). Quercetin and quercetin glucosides were assayed in onion bulbs before and after the aging process, using high performance liquid chromatography-electrospray ion trap mass spectrometry (HPLC-ESI/MS/MS). Quercetin mono- and diglucosides were identified in fresh onion bulbs, whereas quercetin aglycone was the only form present in aged black onion bulbs. These findings indicate that the quercetin mono- and di-glucosides present in fresh onions undergo complete deglycosylation during the aging process. Such profiling will provide a rapid method that can be used to assess changes in the two major quercetin glycosides during the aging process of onion bulbs
Keyword
Allium cepaflavonolquercetinquercetin glucosideHPLC-ESI/MS/MS
ISSN
I000-0052
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5352/JLS.2011.21.3.464
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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