Effect of NaCl on hydrolytic activity of leucine aminopeptidase from Bacillus sp. N2 = Bacillus sp. N2 유래 leucine aminopeptidase의 가수분해활성에 대한 NaCl의 영향

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dc.contributor.authorDong Min Chung-
dc.contributor.authorG D Lee-
dc.contributor.authorS S Chun-
dc.contributor.authorY C Chung-
dc.contributor.authorHyo Kon Chun-
dc.date.accessioned2017-04-19T09:25:25Z-
dc.date.available2017-04-19T09:25:25Z-
dc.date.issued2011-
dc.identifier.issnI000-0052-
dc.identifier.uri10.5352/JLS.2011.21.5.761ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10335-
dc.description.abstractSalt stability of enzymes is a crucial practical factor in the food industry. Previously, leucine aminopeptidase (LAP) was purified from Bacillus sp. N2. Here, we present the salt effect of LAP using synthetic substrates. LAP had a hydrolytic activity for L-leucine-ρ-nitroanilide in high concentrations of NaCl (up to 4 M), but not for other neutral salts (LiBr, LiCl, NaBr, KBr, and KCl). It hydrolyzed various synthetic di-peptide substrates with hydrophobic and hydrophilic amino acids at the C-terminal Xaa region, in the presence of 0-4 M NaCl. The result indicated that the hydrolytic action of LAP is not dependent on the hydrophobicity of the amino acid side chain at the scissile bond of the substrate. Remarkably, the hydrolytic activity of LAP was 1-3 folds higher than those of other LAPs and aminopeptidases in 4.5 M NaCl, suggesting that NaCl-tolerant LAP might be used in the food industry as cheese and anchovy sauce.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleEffect of NaCl on hydrolytic activity of leucine aminopeptidase from Bacillus sp. N2 = Bacillus sp. N2 유래 leucine aminopeptidase의 가수분해활성에 대한 NaCl의 영향-
dc.title.alternativeEffect of NaCl on hydrolytic activity of leucine aminopeptidase from Bacillus sp. N2-
dc.typeArticle-
dc.citation.titleKorean Journal of Life Sciences-
dc.citation.number5-
dc.citation.endPage765-
dc.citation.startPage761-
dc.citation.volume21-
dc.contributor.affiliatedAuthorDong Min Chung-
dc.contributor.affiliatedAuthorHyo Kon Chun-
dc.contributor.alternativeName정동민-
dc.contributor.alternativeName이강덕-
dc.contributor.alternativeName전성식-
dc.contributor.alternativeName정영철-
dc.contributor.alternativeName전효곤-
dc.identifier.bibliographicCitationKorean Journal of Life Sciences, vol. 21, no. 5, pp. 761-765-
dc.identifier.doi10.5352/JLS.2011.21.5.761-
dc.subject.keywordAmino acid-
dc.subject.keywordBacillus sp.-
dc.subject.keywordexopeptidase-
dc.subject.keywordleucine aminopeptidase-
dc.subject.keywordsalt-
dc.subject.localAmino acid-
dc.subject.localamino acid-
dc.subject.localAmino acids-
dc.subject.localBacillus sp-
dc.subject.localbacillus sp.-
dc.subject.localBacillus sp.-
dc.subject.localexopeptidase-
dc.subject.localleucine aminopeptidase-
dc.subject.localSalt-
dc.subject.localsalt-
dc.description.journalClassN-
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Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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