NADPH-dependent pgi-gene knockout Escherichia coli metabolism producing shikimate on different carbon sources = pgi 결손 대장균에서의 쉬큄산 생산

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Title
NADPH-dependent pgi-gene knockout Escherichia coli metabolism producing shikimate on different carbon sources = pgi 결손 대장균에서의 쉬큄산 생산
Author(s)
Jungoh Ahn; B K S Chung; D Y Lee; M Park; I A Karimi; Joon Ki Jung; Hong-Weon Lee
Bibliographic Citation
FEMS Microbiology Letters, vol. 324, no. 1, pp. 10-16
Publication Year
2011
Abstract
We explored the physiological and metabolic effects of different carbon sources (glucose, fructose, and glucose/fructose mixture) in phosphoglucose isomerase (pgi) knockout Escherichia coli mutant producing shikimic acid (SA). It was observed that the pgi- mutant grown on glucose exhibited significantly lower cell growth compared with the pgi+ strain and its mixed glucose/fructose fermentation grew well. Interestingly, when fructose was used as a carbon source, the pgi- mutant showed the enhanced SA production compared with the pgi+ strain. In silico analysis of a genome-scale E. coli model was then conducted to characterize the cellular metabolism and quantify NAPDH regeneration, which allowed us to understand such experimentally observed attenuated cell growth and enhanced SA production in glucose- and fructose-consuming pgi- mutant, respectively with respect to cofactor regeneration.
Keyword
Escherichia coliGenome-scale in silico modelNADPH regenerationPhosphoglucose isomeraseShikimic acid
ISSN
0378-1097
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1111/j.1574-6968.2011.02378.x
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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