Quantitative analysis of transcript variants of CHM gene containing LTR12C element in humans = LTR12C를 포함하는 CHM 유전자의 다양한 전사체의 정량적 분석

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Title
Quantitative analysis of transcript variants of CHM gene containing LTR12C element in humans = LTR12C를 포함하는 CHM 유전자의 다양한 전사체의 정량적 분석
Author(s)
Y D Jung; Jae Won HuhDae Soo Kim; Y J Kim; K Ahn; H S Ha; J R Lee; J M Yi; J W Moon; T O Kim; G A Song; K Han; H S Kim
Bibliographic Citation
Gene, vol. 489, no. 1, pp. 1-5
Publication Year
2011
Abstract
Choroideremia (CHM) is essential for the posttranslational activation of retina-specific Rab protein. Transcript variants (a and b) of the CHM gene were detected in human cancer cells and tissues. Sequence analysis of the both variants found that isoform b is caused by an LTR12C element offering an alternative splicing site within the CHM gene. We performed real-time RT-PCR analysis to study expression levels of the CHM transcript variants in tumor and adjacent normal tissue samples. Our results showed that CHM isoform b was highly expressed in tumor tissues but its expression levels were relatively low in those of adjacent normal tissues including colon, testis, and lung. In addition, high expression levels of CHM isoform b were detected in the cancer cell lines of colon and lung, and colon cancer patient tissues. Thus, we suggest that expression levels of alternative transcripts of the CHM gene could be used as a molecular marker system to identify human cancers.
Keyword
Alternative splicingChoroideremia (CHM)ExpressionLong terminal repeats (LTRs)
ISSN
0378-1119
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.gene.2011.09.001
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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