Bacillus rhizosphaerae sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil

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Title
Bacillus rhizosphaerae sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil
Author(s)
M Madhaiyan; S Poonguzhali; Jung-Sook Lee; Keun Chul Lee; K Hari
Bibliographic Citation
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, vol. 100, no. 3, pp. 437-444
Publication Year
2011
Abstract
A Gram-positive, non-pigmented, rod-shaped, diazotrophic bacterial strain, designated SC-N012 T, was isolated from rhizosphere soil of sugarcane and was subjected to a polyphasic taxonomic study. The strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Bacillus. Sequence analysis of the 16S rRNA gene of SC-N012 T revealed the closest match (98.9% pair wise similarity) with Bacillus clausii DSM 8716 T. However, DNA-DNA hybridization experiments indicated low levels of genomic relatedness (32%) with this strain. The major components of the fatty acid profile are iso-C 15:0, anteiso-C 15:0, iso-C 17:0 and anteiso-C 17:0. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. The G+C content of the genomic DNA is 43.0 mol%. The lipids present in strain SC-N012 T are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol and two unknown phospholipids. Their predominant respiratory quinone was MK-7. Studies of DNA-DNA relatedness, morphological, physiological and chemotaxonomic analyses and phylogenetic data based on 16S rRNA gene sequencing allowed strain SC-N012 T to be described as members of novel species of the genus Bacillus, for which the name Bacillus rhizosphaerae sp. nov. is proposed. The type strain is SC-N012 T (=DSM 21911 T = NCCB 100267 T).
Keyword
Bacillus rhizosphaerae sp. nov. 16S rRNAN-fixation
ISSN
0003-6072
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10482-011-9600-3
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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