Production of recombinant human keratinocyte growth factor from Bombyx mori (Lepidopera: Bombycidae) Bm5 cells = 누에 배양세포에서 인간형 재조합단백질 각질세포 성장인자 생산

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorS Y Han-
dc.contributor.authorC Y Jin-
dc.contributor.authorK Kwon-
dc.contributor.authorE Y Yun-
dc.contributor.authorT W Goo-
dc.contributor.authorS W Kim-
dc.contributor.authorJ S Choi-
dc.contributor.authorKweon Yu-
dc.contributor.authorO Y Kwon-
dc.date.accessioned2017-04-19T09:25:47Z-
dc.date.available2017-04-19T09:25:47Z-
dc.date.issued2011-
dc.identifier.issnI0000128-
dc.identifier.uri10.5352/JLS.2011.21.6.907ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10393-
dc.description.abstractUsing silkworm Bombyx mori Bm5 cells, we established a stable cell line expressing the human keratinocyte growth factor (hKGF), named by the Bm5-hKGF cell, in which the protein hKGF is synthesized in the cell and secreted in the cell culture supernatant (CCS) at approximately 15-20 ng/ml. When the Bm5-hKGF cell was co-expressed with B. mori protein disulfide isomerase (bPDI) cDNA, its secretion increased by about two times the original amount. Through wound healing migration assay, it was demonstrated that the secreted hKGF included in the CCS has a very powerful biological activity of keratinocyte proliferation. We expect to produce useful human recombinant proteins from silkworm cultured cells in large quantities at low prices.-
dc.publisherSouth Korea-
dc.titleProduction of recombinant human keratinocyte growth factor from Bombyx mori (Lepidopera: Bombycidae) Bm5 cells = 누에 배양세포에서 인간형 재조합단백질 각질세포 성장인자 생산-
dc.title.alternativeProduction of recombinant human keratinocyte growth factor from Bombyx mori (Lepidopera: Bombycidae) Bm5 cells-
dc.typeArticle-
dc.citation.titleJournal of Life Science-
dc.citation.number6-
dc.citation.endPage911-
dc.citation.startPage907-
dc.citation.volume21-
dc.contributor.affiliatedAuthorKweon Yu-
dc.contributor.alternativeName한송이-
dc.contributor.alternativeName진초이-
dc.contributor.alternativeName권기상-
dc.contributor.alternativeName윤은영-
dc.contributor.alternativeName구태원-
dc.contributor.alternativeName김승환-
dc.contributor.alternativeName최종순-
dc.contributor.alternativeName유권-
dc.contributor.alternativeName권오유-
dc.identifier.bibliographicCitationJournal of Life Science, vol. 21, no. 6, pp. 907-911-
dc.identifier.doi10.5352/JLS.2011.21.6.907-
dc.subject.keywordSilkworm Bombyx mori Bm5 cells-
dc.subject.keywordhuman keratinocyte growth factor-
dc.subject.keywordprotein disulfide isomerase-
dc.subject.localSilkworm Bombyx mori Bm5 cells-
dc.subject.localSilkworm bombyx mori bm5 cells-
dc.subject.localhuman keratinocyte growth factor-
dc.subject.localprotein disulfide isomerase-
dc.subject.localProtein disulfide isomerase (PDI)-
dc.description.journalClassN-
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.