Host-dependent suppression of RNA silencing mediated by the viral suppressor p19 in potato

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Title
Host-dependent suppression of RNA silencing mediated by the viral suppressor p19 in potato
Author(s)
Joon Woo Ahn; Jong Suk Lee; S J Davarpanah; Jae Heung Jeon; Y I Park; Jang Ryol Liu; Won Joong Jeong
Bibliographic Citation
Planta, vol. 234, no. 5, pp. 1065-1072
Publication Year
2011
Abstract
p19 protein encoded by tomato bushy stunt virus (TBSV) is known as a suppressor of RNA silencing via inhibition of small RNA-guided cleavage in plants. In this study, we generated TBSVp19-expressing patatin-RNAi transgenic potatoes to identify the inhibitory mechanisms of RNA silencing mediated by TBSVp19. In TBSVp19-expressing patatin-RNAi lines, reduction of patatin-derived siRNA accumulation and complementation of patatin transcripts were detected in comparison with the non-TBSVp19-expressing patatin-RNAi line, suggesting that TBSVp19 suppresses the siRNA-mediated silencing pathway. Interestingly, no apparent effect on the accumulation of miRNA168 and other miRNAs was detected in TBSVp19-expressing lines; previous studies reported that p19 induced the accumulation of both miRNA168 and its target Argonaute 1 (AGO1) mRNA, but suppressed AGO1 translation via up-regulation of miRNA168 in Arabidopsis. In addition, the expression of Argonaute 1 (AGO1-1 and AGO1-2) and Dicer-like 1 (DCL1) was not significantly altered in p19-expressing lines. Interestingly, no translational inhibition of AGO1 mediated by p19 was detected. These results suggest that p19 suppresses siRNA-mediated silencing in potato, but may not affect miRNA-mediated silencing, possibly due to the host-dependent manner of p19 activity.
Keyword
MicroRNA (miRNA)RNA silencingRNA-induced silencing complexes (RISCs)Short interfering RNA (siRNA)Tomato bushy stunt virus p19 (TBSVp19)
ISSN
0032-0935
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00425-011-1465-1
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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