Mouse model for hemolytic uremic syndrome induced by outer membrane vesicles of Escherichia coli O157:H7 = 대장균 O157 OMV에 의한 마우스 모델 용혈성뇨독증후군(HUS)의 유도

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Title
Mouse model for hemolytic uremic syndrome induced by outer membrane vesicles of Escherichia coli O157:H7 = 대장균 O157 OMV에 의한 마우스 모델 용혈성뇨독증후군(HUS)의 유도
Author(s)
Sang-Hyun Kim; Yong Hoon Lee; Sang Ho Lee; Sang Rae LeeJae Won HuhSun-Uk Kim; Kyu Tae Chang
Bibliographic Citation
FEMS Immunology and Medical Microbiology, vol. 63, no. 3, pp. 427-434
Publication Year
2011
Abstract
Hemolytic uremic syndrome (HUS) is characterized by acute renal failure in children and is typically complicated with thrombocytopenia and hemolytic anemia. Although mouse models of HUS have been evaluated using Shiga toxin (STx) combined with or without lipopolysaccharide (LPS), no HUS model has been tested using purified outer membrane vesicles (OMVs) from STx-producing Escherichia coli (STEC) O157:H7. Accordingly, we investigated whether OMVs of STEC O157:H7 conveying STx2 and LPS can cause HUS-like symptoms in mice inoculated intraperitoneally. Three types of OMVs differing in LPS acylation status and STx2 amount were used to compare their ability to induce HUS-like symptoms. Native OMVs (nOMV) with fully hexa-acylated LPS caused HUS-like symptoms at 72-96 h after dually divided injections of 1 μg nOMV per animal. However, elevated doses of modified OMVs (mOMV) carrying mainly penta-acylated LPS were required to induce similar HUS signs. Moreover, mitomycin-C-induced OMVs (mcOMV) that were enriched with STx2 induced HUS-like symptoms similar to those of nOMV at the same dose. These results suggest that the OMVs of STEC O157:H7 potentiated with STx2 and fully hexa-acylated LPS can be utilized for inducing HUS-like symptoms in mice and could be the causative material involved in the development of HUS.
Keyword
Hemolytic uremic syndromeLipopolysaccharideOuter membrane vesiclesShiga toxin
ISSN
0928-8244
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1111/j.1574-695X.2011.00869.x
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
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