DC Field | Value | Language |
---|---|---|
dc.contributor.author | H A Jang | - |
dc.contributor.author | Hyun A Kim | - |
dc.contributor.author | Suk Yoon Kwon | - |
dc.contributor.author | D W Choi | - |
dc.contributor.author | P S Choi | - |
dc.date.accessioned | 2017-04-19T09:25:57Z | - |
dc.date.available | 2017-04-19T09:25:57Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1229-2818 | - |
dc.identifier.uri | 10.5010/JPB.2011.38.3.198 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10409 | - |
dc.description.abstract | Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic cucumber. Cotyledonary-node explants of cucumber (Cucumis sativus L. cv., Eunsung) were co-cultivated with Agrobacterium strains (EHA101) containing the binary vector (pPZP211) carrying with CaMV 35S promoter-nptII gene as selectable marker gene and 35S promoter-DQ gene (unpublished data) as target gene. The average of transformation efficiency (4.01%) was obtained from three times experiments and the maximum efficiency was shown at 5.97%. A total of 9 putative transgenic plants resistant to paromomycin were produced from the cultures of cotyledonary-node explants on selection medium. Among them, 6 transgenic plants showed that the nptII gene integrated into each genome of cucumber by Southern blot analysis. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | The use of cotyledonary-node explants in Agrobacterium tumefaciens-mediated transformation of cucumber (Cucumis sativus L.) = Agrobacterium에 의한 오이 형질전환에서 자엽절 절편의 이용 | - |
dc.title.alternative | The use of cotyledonary-node explants in Agrobacterium tumefaciens-mediated transformation of cucumber (Cucumis sativus L.) | - |
dc.type | Article | - |
dc.citation.title | Journal of Plant Biotechnology | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 202 | - |
dc.citation.startPage | 198 | - |
dc.citation.volume | 38 | - |
dc.contributor.affiliatedAuthor | Hyun A Kim | - |
dc.contributor.affiliatedAuthor | Suk Yoon Kwon | - |
dc.contributor.alternativeName | 장현아 | - |
dc.contributor.alternativeName | 김현아 | - |
dc.contributor.alternativeName | 권석윤 | - |
dc.contributor.alternativeName | 최동욱 | - |
dc.contributor.alternativeName | 최필선 | - |
dc.identifier.bibliographicCitation | Journal of Plant Biotechnology, vol. 38, no. 3, pp. 198-202 | - |
dc.identifier.doi | 10.5010/JPB.2011.38.3.198 | - |
dc.subject.keyword | Agrobacterium | - |
dc.subject.keyword | cotyledonary-node | - |
dc.subject.keyword | paromomycin | - |
dc.subject.keyword | transgenic cucumber | - |
dc.subject.local | Agrobacterium | - |
dc.subject.local | agrobacterium | - |
dc.subject.local | Agrobact erium | - |
dc.subject.local | cotyledonary-node | - |
dc.subject.local | cotyledonary node | - |
dc.subject.local | paromomycin | - |
dc.subject.local | Paromomycin | - |
dc.subject.local | Transgenic cucumber | - |
dc.subject.local | transgenic cucumber | - |
dc.description.journalClass | N | - |
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