The use of cotyledonary-node explants in Agrobacterium tumefaciens-mediated transformation of cucumber (Cucumis sativus L.) = Agrobacterium에 의한 오이 형질전환에서 자엽절 절편의 이용

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dc.contributor.authorH A Jang-
dc.contributor.authorHyun A Kim-
dc.contributor.authorSuk Yoon Kwon-
dc.contributor.authorD W Choi-
dc.contributor.authorP S Choi-
dc.date.accessioned2017-04-19T09:25:57Z-
dc.date.available2017-04-19T09:25:57Z-
dc.date.issued2011-
dc.identifier.issn12292818-
dc.identifier.uri10.5010/JPB.2011.38.3.198ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10409-
dc.description.abstractAgrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic cucumber. Cotyledonary-node explants of cucumber (Cucumis sativus L. cv., Eunsung) were co-cultivated with Agrobacterium strains (EHA101) containing the binary vector (pPZP211) carrying with CaMV 35S promoter-nptII gene as selectable marker gene and 35S promoter-DQ gene (unpublished data) as target gene. The average of transformation efficiency (4.01%) was obtained from three times experiments and the maximum efficiency was shown at 5.97%. A total of 9 putative transgenic plants resistant to paromomycin were produced from the cultures of cotyledonary-node explants on selection medium. Among them, 6 transgenic plants showed that the nptII gene integrated into each genome of cucumber by Southern blot analysis.-
dc.publisherSouth Korea-
dc.titleThe use of cotyledonary-node explants in Agrobacterium tumefaciens-mediated transformation of cucumber (Cucumis sativus L.) = Agrobacterium에 의한 오이 형질전환에서 자엽절 절편의 이용-
dc.title.alternativeThe use of cotyledonary-node explants in Agrobacterium tumefaciens-mediated transformation of cucumber (Cucumis sativus L.)-
dc.typeArticle-
dc.citation.titleJournal of Plant Biotechnology-
dc.citation.number3-
dc.citation.endPage202-
dc.citation.startPage198-
dc.citation.volume38-
dc.contributor.affiliatedAuthorSuk Yoon Kwon-
dc.contributor.alternativeName장현아-
dc.contributor.alternativeName김현아-
dc.contributor.alternativeName권석윤-
dc.contributor.alternativeName최동욱-
dc.contributor.alternativeName최필선-
dc.identifier.bibliographicCitationJournal of Plant Biotechnology, vol. 38, no. 3, pp. 198-202-
dc.identifier.doi10.5010/JPB.2011.38.3.198-
dc.subject.keywordAgrobacterium-
dc.subject.keywordcotyledonary-node-
dc.subject.keywordparomomycin-
dc.subject.keywordtransgenic cucumber-
dc.subject.localAgrobacterium-
dc.subject.localcotyledonary-node-
dc.subject.localcotyledonary node-
dc.subject.localparomomycin-
dc.subject.localParomomycin-
dc.subject.localtransgenic cucumber-
dc.subject.localTransgenic cucumber-
dc.description.journalClassN-
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Division of Biomaterials Research > 1. Journal Articles
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