Effect of nitrogen limitation on oleic acid biosynthesis in Botryococcus braunii

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Title
Effect of nitrogen limitation on oleic acid biosynthesis in Botryococcus braunii
Author(s)
Gang Guk Choi; Byung Hyuk Kim; Chi-Yong AhnHee-Mock Oh
Bibliographic Citation
Journal of Applied Phycology, vol. 23, no. 6, pp. 1031-1037
Publication Year
2011
Abstract
The influence of nitrogen (N) deficiency on the cell growth and intracellular lipid production of the alga Botryococcus braunii UTEX 572 was investigated. Biomass concentration and lipid content of B. braunii cultivated in modified Chu-13 medium containing 0.04, 0.37, and 3.66 mM nitrate were 0.23-0.38 g L-1 and 36-63% of dry cell weight, respectively. The specific growth rate of B. braunii reached a constant of 0.185 day-1 during cultivation with an initial nitrate feed of 3.66 mM. The maximum lipid content of B. braunii was 63% with 0.04 mM nitrate. However, the maximum lipid productivity of 0.019 g L-1 day-1 was achieved with 0.37 mM nitrate. The level of oleic acid, an important component of biodiesel, was higher at 86% of the total fatty acids under N-limited conditions (0.04 mM nitrate) compared to 69% under N-sufficient conditions (3.66 mM nitrate). Furthermore, expression of the stearoyl-ACP desaturase gene (sad) encoding a stearoyl-ACP desaturase involved in the synthesis of oleic acid was 2. 6-fold higher under N-limited conditions than under N-sufficient conditions.
Keyword
BiodieselBotryococcus brauniiNitrogen limitationOleic acidsadStearoyl-ACP desaturase
ISSN
0921-8971
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10811-010-9636-1
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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