Molecular analysis of the luminal and mucosal-associated intestinal microbiota in diarrhea-predominant irritable bowel syndrome

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dc.contributor.authorI M Carroll-
dc.contributor.authorT Ringel-Kulka-
dc.contributor.authorT O Keku-
dc.contributor.authorYoung Hyo Chang-
dc.contributor.authorC D Packey-
dc.contributor.authorR B Sartor-
dc.contributor.authorY Ringel-
dc.date.accessioned2017-04-19T09:26:21Z-
dc.date.available2017-04-19T09:26:21Z-
dc.date.issued2011-
dc.identifier.issn0193-1857-
dc.identifier.uri10.1152/ajpgi.00154.2011ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10431-
dc.description.abstractAlterations in the intestinal microbiota have been suggested as an etiological factor in the pathogenesis of irritable bowel syndrome (IBS). This study used a molecular fingerprinting technique to compare the composition and biodiversity of the microbiota within fecal and mucosal niches between patients with diarrhea-predominant IBS (D-IBS) and healthy controls. Terminal-restriction fragment (T-RF) length polymorphism (T-RFLP) fingerprinting of the bacterial 16S rRNA gene was used to perform microbial community composition analyses on fecal and mucosal samples from patients with D-IBS (n = 16) and healthy controls (n = 21). Molecular fingerprinting of the microbiota from fecal and colonic mucosal samples revealed differences in the contribution of T-RFs to the microbiota between D-IBS patients and healthy controls. Further analysis revealed a significantly lower (1.2-fold) biodiversity of microbes within fecal samples from D-IBS patients than healthy controls (P = 0.008). No difference in biodiversity in mucosal samples was detected between D-IBS patients and healthy controls. Multivariate analysis of T-RFLP profiles demonstrated distinct microbial communities between luminal and mucosal niches in all samples. Our findings of compositional differences in the luminal- and mucosal-associated microbiota between D-IBS patients and healthy controls and diminished microbial biodiversity in D-IBS fecal samples further support the hypothesis that alterations in the intestinal microbiota may have an etiological role in the pathogenesis of D-IBS and suggest that luminal and mucosal niches need to be investigated.-
dc.publisherAmer Physiological Soc-
dc.titleMolecular analysis of the luminal and mucosal-associated intestinal microbiota in diarrhea-predominant irritable bowel syndrome-
dc.title.alternativeMolecular analysis of the luminal and mucosal-associated intestinal microbiota in diarrhea-predominant irritable bowel syndrome-
dc.typeArticle-
dc.citation.titleAmerican Journal of Physiology-Gastrointestinal and Liver Physiology-
dc.citation.number5-
dc.citation.endPageG807-
dc.citation.startPageG799-
dc.citation.volume301-
dc.contributor.affiliatedAuthorYoung Hyo Chang-
dc.contributor.alternativeNameCarroll-
dc.contributor.alternativeNameRingel-Kulka-
dc.contributor.alternativeNameKeku-
dc.contributor.alternativeName장영효-
dc.contributor.alternativeNamePackey-
dc.contributor.alternativeNameSartor-
dc.contributor.alternativeNameRingel-
dc.identifier.bibliographicCitationAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, vol. 301, no. 5, pp. G799-G807-
dc.identifier.doi10.1152/ajpgi.00154.2011-
dc.subject.keywordMicrobial biodiversity-
dc.subject.keywordTerminal-restriction fragment length polymorphism-
dc.subject.localMicrobial biodiversity-
dc.subject.localTerminal-restriction fragment length polymorphism-
dc.description.journalClassY-
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Ochang Branch Institute > Division of National Bio-Infrastructure > Bio-Infrastructure Policy Support Center > 1. Journal Articles
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