DC Field | Value | Language |
---|---|---|
dc.contributor.author | L H Luo | - |
dc.contributor.author | Chul Ho Kim | - |
dc.contributor.author | Sun-Yeon Heo | - |
dc.contributor.author | Baek Rock Oh | - |
dc.contributor.author | Won Kyung Hong | - |
dc.contributor.author | Seonghun Kim | - |
dc.contributor.author | D H Kim | - |
dc.contributor.author | Jeong-Woo Seo | - |
dc.date.accessioned | 2017-04-19T09:26:21Z | - |
dc.date.available | 2017-04-19T09:26:21Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | 10.1016/j.biortech.2011.09.099 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10434 | - |
dc.description.abstract | The pduP gene encodes a propionaldehyde dehydrogenase (PduP) was investigated for the role in 3-hydroxypropionic acid (3-HP) glycerol metabolism in Klebsiella pneumoniae. The enzyme assay showed that cell extracts from a pduP mutant strain lacked measurable dehydrogenase activity. Additionally, the mutant strain accumulated the cytotoxic intermediate metabolite 3-hydroxypropionaldehyde (3-HPA), causing both cell death and a lower final 3-HP titer. Ectopic expression of pduP restored normal cell growth to mutant. The enzymatic property of recombinant protein from Escherichia coli was examined, exhibiting a broad substrate specificity, being active on 3-HPA. The present work is thus the first to demonstrate the role of PduP in glycerol metabolism and biosynthesis of 3-HP. | - |
dc.publisher | Elsevier | - |
dc.title | Production of 3-hydroxypropionic acid through propionaldehyde dehydrogenase PduP mediated biosynthetic pathway in Klebsiella penumoniae | - |
dc.title.alternative | Production of 3-hydroxypropionic acid through propionaldehyde dehydrogenase PduP mediated biosynthetic pathway in Klebsiella penumoniae | - |
dc.type | Article | - |
dc.citation.title | Bioresource Technology | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 6 | - |
dc.citation.startPage | 1 | - |
dc.citation.volume | 103 | - |
dc.contributor.affiliatedAuthor | Chul Ho Kim | - |
dc.contributor.affiliatedAuthor | Sun-Yeon Heo | - |
dc.contributor.affiliatedAuthor | Baek Rock Oh | - |
dc.contributor.affiliatedAuthor | Won Kyung Hong | - |
dc.contributor.affiliatedAuthor | Seonghun Kim | - |
dc.contributor.affiliatedAuthor | Jeong-Woo Seo | - |
dc.contributor.alternativeName | Luo | - |
dc.contributor.alternativeName | 김철호 | - |
dc.contributor.alternativeName | 허선연 | - |
dc.contributor.alternativeName | 오백록 | - |
dc.contributor.alternativeName | 홍원경 | - |
dc.contributor.alternativeName | 김성훈 | - |
dc.contributor.alternativeName | 김대혁 | - |
dc.contributor.alternativeName | 서정우 | - |
dc.identifier.bibliographicCitation | Bioresource Technology, vol. 103, no. 1, pp. 1-6 | - |
dc.identifier.doi | 10.1016/j.biortech.2011.09.099 | - |
dc.subject.keyword | 3-hydroxypropionic acid | - |
dc.subject.keyword | Glycerol | - |
dc.subject.keyword | Klebsiella pneumoniae | - |
dc.subject.keyword | Propanediol utilization protein | - |
dc.subject.keyword | Propionaldehyde dehydrogenase | - |
dc.subject.local | 3-Hydroxypropionic acid | - |
dc.subject.local | 3-hydroxypropionic acid | - |
dc.subject.local | Glycerol | - |
dc.subject.local | glycerol | - |
dc.subject.local | Klebsiella pneumonia | - |
dc.subject.local | Klebsiella pneumoniae | - |
dc.subject.local | Propanediol utilization protein PduP | - |
dc.subject.local | Propanediol utilization protein | - |
dc.subject.local | Propionaldehyde dehydrogenase | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.