Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor = 형질전한 좀개구리밥에서 PEDV antigen 발현

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Title
Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor = 형질전한 좀개구리밥에서 PEDV antigen 발현
Author(s)
S M Ko; H J Sun; Myung Jin Oh; I J Song; M J Kim; H S Sin; C H Goh; Y W Kim; P O Lim; H Y Lee; Suk Weon Kim
Bibliographic Citation
Horticulture Environment and Biotechnology, vol. 52, no. 5, pp. 511-515
Publication Year
2011
Abstract
Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.
Keyword
aquatic plantporcine diarrheaspike proteintransformationvaccine
ISSN
0253-6498
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1007/s13580-011-0007-x
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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