RPTPu tyrosine phopshatase promotes adipogenic differentiation via modultion of p120 catenin phosphoryltion

Cited 21 time in scopus
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Title
RPTPu tyrosine phopshatase promotes adipogenic differentiation via modultion of p120 catenin phosphoryltion
Author(s)
Won Kon-Kim; Hyeyun Jung; Eun Young Kim; Do Hyung Kim; Yee Sook ChoByoung Chul ParkSung Goo Park; Y Ko; Kwang-Hee BaeSang Chul Lee
Bibliographic Citation
Molecular Biology of Cell, vol. 22, no. 24, pp. 4883-4891
Publication Year
2011
Abstract
Adipocyte differentiation can be regulated by the combined activity of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs). In particular, PTPs act as key regulators in differentiation-associated signaling pathways. We recently found that receptor-type PTPμ (RPTPμ) expression is markedly increased during the adipogenic differentiation of 3T3-L1 preadipocytes and mesenchymal stem cells. Here, we investigate the functional roles of RPTPμ and the mechanism of its involvement in the regulation of signal transduction during adipogenesis of 3T3-L1 cells. Depletion of endogenous RPTPμ by RNA interference significantly inhibited adipogenic differentiation, whereas RPTPμ overexpression led to an increase in adipogenic differentiation. Ectopic expression of p120 catenin suppressed adipocyte differentiation, and the decrease in adipogenesis by p120 catenin was recovered by introducing RPTPμ. Moreover, RPTPμ induced a decrease in the cytoplasmic p120 catenin expression by reducing its tyrosine phosphorylation level, consequently leading to enhanced translocation of Glut-4 to the plasma membrane. On the basis of these results, we propose that RPTPμ acts as a positive regulator of adipogenesis by modulating the cytoplasmic p120 catenin level. Our data conclusively demonstrate that differentiation into adipocytes is controlled by RPTPμ, supporting the utility of RPTPμ and p120 catenin as novel target proteins for the treatment of obesity.
ISSN
1059-1524
Publisher
Amer Soc Cell Biology
DOI
http://dx.doi.org/10.1091/mbc.E11-03-0175
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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