Rhodanobacter panaciterrae sp. nov., a bacterium with ginsenoside-converting activity isolated from soil of a ginseng field

Abstract

A novel gammaproteobacterium, designated LnR5-47 T, was isolated from soil of a ginseng field in Liaoning province, China. The isolate was a Gram-negative, aerobic, non-motile, non-sporeforming rod. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LnR5-47 T belonged to the genus Rhodanobacter. The isolate was most closely related to Rhodanobacter ginsengisoli GR17-7 T, Rhodanobacter terrae GP18-1 T, Dyella ginsengisoli Gsoil 3046 T, Rhodanobacter soli DCY45 T, Dyella soli JS12-10 T and Dyella japonica IAM 15069 T (98.0, 97.9, 97.7, 97.3, 97.2 and 97.1% 16S rRNA gene sequence similarity, respectively). Chemotaxonomic data (Q-8 as the predominant ubiquinone, and iso-C16: 0, iso-C17: 1v9c and iso-C15: 0 as the major fatty acids) also supported the affiliation of strain LnR5-47 T with the genus Rhodanobacter. However, DNA-DNA relatedness between strain LnR5-47 T and its closest phylogenetic neighbours was,25.8%. Moreover, physiological and biochemical tests phenotypically differentiated the isolate from other members of the genus Rhodanobacter. Therefore, strain LnR5-47 T represents a novel species, for which the name Rhodanobacter panaciterrae sp. nov. is proposed; the type strain is LnR5-47 T (5KACC 12826 T5KCTC 22232 T5LMG 24460 T).