Two-temperature hybridization for microarray detection of label-free microRNAs with attomole detection and superior specificity = 두 가지 온도를 이용한 마이크로 RNA 어레이 분석

Cited 91 time in scopus
Metadata Downloads
Title
Two-temperature hybridization for microarray detection of label-free microRNAs with attomole detection and superior specificity = 두 가지 온도를 이용한 마이크로 RNA 어레이 분석
Author(s)
Jeong Min Lee; Yongwon Jung
Bibliographic Citation
Angewandte Chemie-International Edition, vol. 50, no. 52, pp. 12487-12490
Publication Year
2011
Abstract
Two is better than one: Two short locked nucleic acid based probes were used to collectively capture and detect microRNAs by a simple two-temperature hybridization process. Intact microRNAs were directly measured down to attomolar concentrations with a high specificity and nearly four orders of magnitude of dynamic range. Single base mismatches in the microRNAs were potently discriminated from the perfectly matched targets.
Keyword
biosensorsLNAsmicroarraysmicroRNAsRNA recognition
ISSN
1433-7851
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/anie.201105605
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.