Arabidopsis TTR1 causes LRR-dependent lethal systemic necrosis, rather than systemic acquired resistance, to Tobacco ringspot virus

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dc.contributor.authorMoon Nam-
dc.contributor.authorSerry Koh-
dc.contributor.authorSung Uk Kim-
dc.contributor.authorL L Domier-
dc.contributor.authorJae Heung Jeon-
dc.contributor.authorH G Kim-
dc.contributor.authorS H Lee-
dc.contributor.authorA F Bent-
dc.contributor.authorJae Sun Moon-
dc.date.accessioned2017-04-19T09:27:22Z-
dc.date.available2017-04-19T09:27:22Z-
dc.date.issued2011-
dc.identifier.issn10168478-
dc.identifier.uri10.1007/s10059-011-0101-zko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10469-
dc.description.abstractMost Arabidopsis ecotypes display tolerance to the Tobacco ringspot virus (TRSV), but a subset of Arabidopsis ecotypes, including Estland (Est), develop lethal systemic necrosis (LSN), which differs from the localized hypersensitive responses (HRs) or systemic acquired resistance (SAR) characteristic of incompatible reactions. Neither viral replication nor the systemic movement of TRSV was restricted in tolerant or sensitive Arabidopsis ecotypes; therefore, the LSN phenotype shown in the sensitive ecotypes might not be due to viral accumulation. In the present study, we identified the Est TTR1 gene (tolerance to Tobacco ringspot virus 1) encoding a TIR-NBS-LRR protein that controls the ecotype-dependent tolerant/sensitive phenotypes by a map-based cloning method. The tolerant Col-0 ecotype Arabidopsis transformed with the sensitive Est TTR1 allele developed an LSN phenotype upon TRSV infection, suggesting that the Est TTR1 allele is dominant over the tolerant ttr1 allele of Col-0. Multiple sequence alignments of 10 tolerant ecotypes from those of eight sensitive ecotypes showed that 10 LRR amino acid polymorphisms were consistently distributed across the TTR1/ttr1 alleles. Site-directed mutagenesis of these amino acids in the LRR region revealed that two sites, L956S and K1124Q, completely abolished the LSN phenotype. VIGS study revealed that TTR1 is dependent on SGT1, rather than EDS1. The LSN phenotype by TTR1 was shown to be transferred to Nicotiana benthamiana, demonstrating functional conservation of TTR1 across plant families, which are involved in SGT-dependent defense responses, rather than EDS1-dependent signaling pathways.-
dc.publisherSouth Korea-
dc.titleArabidopsis TTR1 causes LRR-dependent lethal systemic necrosis, rather than systemic acquired resistance, to Tobacco ringspot virus-
dc.title.alternativeArabidopsis TTR1 causes LRR-dependent lethal systemic necrosis, rather than systemic acquired resistance, to Tobacco ringspot virus-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number5-
dc.citation.endPage429-
dc.citation.startPage421-
dc.citation.volume32-
dc.contributor.affiliatedAuthorJae Heung Jeon-
dc.contributor.affiliatedAuthorJae Sun Moon-
dc.contributor.alternativeName남문-
dc.contributor.alternativeName고세리-
dc.contributor.alternativeName김성욱-
dc.contributor.alternativeNameDomier-
dc.contributor.alternativeName전재흥-
dc.contributor.alternativeName김홍기-
dc.contributor.alternativeName이수헌-
dc.contributor.alternativeNameBent-
dc.contributor.alternativeName문제선-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 32, no. 5, pp. 421-429-
dc.identifier.doi10.1007/s10059-011-0101-z-
dc.subject.keywordArabidopsis-
dc.subject.keywordLethal systemic necrosis-
dc.subject.keywordTIR-NBS-LRR-
dc.subject.keywordTobacco ringspot virus-
dc.subject.keywordTolerance to tobacco ringspot virus 1-
dc.subject.localArabidopsis-
dc.subject.localLethal systemic necrosis-
dc.subject.localTIR-NBS-LRR-
dc.subject.localTobacco ringspot virus-
dc.subject.localTolerance to tobacco ringspot virus 1-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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